Mutation in a flexible linker modulates binding affinity for modular complexes.

Stokes PH, Robertson NO, Silva AP, Estephan T, Trewhella J, Guss JM, Matthews JM
Proteins (2019 Feb 20)

PMID: 30788856 doi: 10.1002/prot.25675

Submitted to SASBDB: 2017 Sep 27 Published in SASBDB: 2019 Mar 1

SASDDQ4 – LIM/homeobox protein Lhx4 LIM domains fused to the LIM interaction domain (LID) of Insulin gene enhancer protein ISL-2 Sample: LIM/homeobox protein Lhx4 monomer, 15 kDa Mus musculus protein Insulin gene enhancer protein ISL-2 monomer, 4 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 19 RgGuinier 2.2 nm Dmax 8.0 nm VolumePorod 20 nm3

SASDDR4 – LIM/homeobox protein Lhx4 LIM domains fused to the LIM interaction domain (LID) R282G mutant of Insulin gene enhancer protein ISL-2 Sample: LIM/homeobox protein Lhx4 monomer, 15 kDa Mus musculus protein Insulin gene enhancer protein ISL-2 (R282G) monomer, 4 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 19 RgGuinier 2.3 nm Dmax 8.5 nm VolumePorod 21 nm3