The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N.

Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH
Biophys J 113(8):1673-1684 (2017 Oct 17)
PMID: 29045862
doi: 10.1016/j.bpj.2017.08.030 		Submitted to SASBDB: 2017 Apr 26
Published in SASBDB:

SASDC23 – Colicin N WT

Colicin N experimental SAS data
Colicin N WT Rg histogram
Sample: Colicin N monomer, 43 kDa Escherichia coli protein
Buffer: 50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2012 Jun 29
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 74 nm3

SASDC33 – Colicin N delta 1-39

Colicin N delta 1-39 experimental SAS data
Colicin N delta 1-39 Rg histogram
Sample: Colicin N delta 1-39 monomer, 39 kDa Escherichia coli protein
Buffer: 50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2012 Jun 29
RgGuinier 2.7 nm
Dmax 9.0 nm
VolumePorod 60 nm3

SASDC43 – Colicin K145A mutant

Colicin N K145A mutant experimental SAS data
Colicin K145A mutant Rg histogram
Sample: Colicin N K145A mutant monomer, 43 kDa Escherichia coli protein
Buffer: 50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2012 Jun 29
RgGuinier 3.2 nm
Dmax 13.6 nm
VolumePorod 68 nm3

SASDC53 – Colicin N Translocation domain

Colicin N Translocation domain experimental SAS data
Colicin N Translocation domain Rg histogram
Sample: Colicin N Translocation domain monomer, 10 kDa Escherichia coli protein
Buffer: 50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2012 Jun 29
RgGuinier 2.8 nm
Dmax 11.4 nm
VolumePorod 22 nm3