Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes.

Bruce HA, Du D, Matak-Vinkovic D, Bandyra KJ, Broadhurst RW, Martin E, Sobott F, Shkumatov AV, Luisi BF
Nucleic Acids Res 46(1):387-402 (2018 Jan 9)

PMID: 29136196 doi: 10.1093/nar/gkx1083

Submitted to SASBDB: 2017 Jun 7 Published in SASBDB: 2017 Nov 9

SASDCY4 – RNase E 603-850 Sample: RNase E 603-850 monomer, 30 kDa Escherichia coli protein Buffer: 50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 5 RgGuinier 5.3 nm Dmax 27.5 nm VolumePorod 139 nm3

SASDCZ4 – RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex Sample: RNase E 603-850 monomer, 30 kDa Escherichia coli protein ATP-dependent RNA helicase RhlB monomer, 47 kDa Escherichia coli protein Buffer: 50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2016 Feb 11 RgGuinier 5.4 nm Dmax 29.5 nm VolumePorod 183 nm3

SASDC25 – RNase E 603-850/ATP-dependent RNA helicase (RhlB)/enolase ternary complex Sample: RNase E 603-850 monomer, 30 kDa Escherichia coli protein ATP-dependent RNA helicase RhlB monomer, 47 kDa Escherichia coli protein Enolase dimer, 91 kDa Escherichia coli protein Buffer: 50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2014 Jul 16 RgGuinier 6.4 nm Dmax 30.5 nm VolumePorod 280 nm3