A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR).

Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
J Biol Chem 287(41):34304-15 (2012 Oct 5)

PMID: 22896701 doi: 10.1074/jbc.M112.398404

Published in SASBDB: 2015 Jul 7

SASDAT4 – uPAR wild-type Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10 RgGuinier 2.8 nm Dmax 9.7 nm VolumePorod 69 nm3

SASDAU4 – uPAR H47C/N259C Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10 RgGuinier 2.2 nm Dmax 7.0 nm VolumePorod 57 nm3

SASDAV4 – uPAR wild-type AE105 complex Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Synthetic peptide AE105 monomer, 1 kDa protein Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10 RgGuinier 2.5 nm Dmax 8.5 nm VolumePorod 61 nm3

SASDAW4 – uPAR wild-type AE234 complex Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Synthetic peptide AE234 monomer, 1 kDa protein Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10 RgGuinier 2.4 nm Dmax 8.3 nm VolumePorod 57 nm3

SASDAX4 – uPAR wild-type ATF complex Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Urokinase-type plasminogen activator monomer, 49 kDa Homo sapiens protein Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10 RgGuinier 2.8 nm Dmax 9.4 nm VolumePorod 102 nm3