Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.

Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M
J Mol Biol 431(19):3787-3803 (2019 Sep 6)

PMID: 31288030 doi: 10.1016/j.jmb.2019.07.004

Submitted to SASBDB: 2018 Nov 2 Published in SASBDB: 2019 Sep 13

SASDEJ7 – The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein Surface presentation of antigens protein SpaO(SPOA1,2) monomer, 34 kDa Salmonella enterica subsp. … protein Oxygen-regulated invasion protein OrgB dimer, 53 kDa Salmonella enterica subsp. … protein ATP synthase InvC monomer, 48 kDa Salmonella enterica subsp. … protein Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 12 RgGuinier 6.0 nm Dmax 22.7 nm VolumePorod 302 nm3

SASDEK7 – SpaO C-terminus Sample: Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus monomer, 19 kDa Salmonella enterica subsp. … protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24 RgGuinier 2.1 nm Dmax 7.2 nm

SASDC68 – Surface presentation of antigens protein, SpaOc Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24 RgGuinier 2.5 nm Dmax 9.1 nm VolumePorod 51 nm3

SASDC78 – Surface presentation of antigens protein SpaOc-SpaO(SPOA1,2) Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein Surface presentation of antigens protein SpaO(SPOA1,2) monomer, 34 kDa Salmonella enterica subsp. … protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24 RgGuinier 3.3 nm Dmax 11.1 nm VolumePorod 108 nm3

SASDC88 – Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus Sample: Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus monomer, 17 kDa Salmonella enterica subsp. … protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24 RgGuinier 1.6 nm Dmax 5.2 nm VolumePorod 31 nm3

SASDC98 – Surface presentation of antigens protein SpaOc-SpaO N-terminus Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus monomer, 17 kDa Salmonella enterica subsp. … protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24 RgGuinier 2.9 nm Dmax 10.6 nm VolumePorod 68 nm3