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31 hits found for Tuukkanen

SASDC32 – Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB)

Ethylene Receptor 1 experimental SAS data
Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB) Rg histogram
Sample: Ethylene Receptor 1 dimer, 129 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris-NDSB 150 mM NaCl 1mM DTT 250 mM NDSB, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Mar 19
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Tuukkanen A, Mertens HD, Mueller-Dieckmann J
RgGuinier 4.7 nm
Dmax 15.8 nm
VolumePorod 316 nm3

SASDC42 – Ethylene Receptor 1 Cytosolic Domain

Ethylene Receptor 1 experimental SAS data
Ethylene Receptor 1 Cytosolic Domain Rg histogram
Sample: Ethylene Receptor 1 dimer, 129 kDa Arabidopsis thaliana protein
Buffer: 20 mM TRIS 150 mM NaCl 1mM DTT 250mM NDSB, pH: 8.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 29
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Tuukkanen A, Mertens HD, Mueller-Dieckmann J
RgGuinier 5.5 nm
Dmax 19.0 nm
VolumePorod 274 nm3

SASDDQ2 – EspG3 chaperone from Mycobacterium marinum

EspG3 chaperone from Mycobacterium marinum M experimental SAS data
EspG3 chaperone from Mycobacterium marinum M Kratky plot
Sample: EspG3 chaperone from Mycobacterium marinum M monomer, 32 kDa Mycobacterium marinum M protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.3 nm
Dmax 8.0 nm

SASDDR2 – EspG1 chaperone from Mycobacterium marinum

EspG1 from Mycobacterium marinum experimental SAS data
EspG1 from Mycobacterium marinum Kratky plot
Sample: EspG1 from Mycobacterium marinum monomer, 30 kDa Mycobacterium marinum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.7 nm
Dmax 9.7 nm

SASDDS2 – EspG3 chaperone from Mycobacter smegmatis

EspG3 chaperone from Mycobacterium smegmatis experimental SAS data
EspG3 chaperone from Mycobacterium smegmatis Kratky plot
Sample: EspG3 chaperone from Mycobacterium smegmatis monomer, 32 kDa Mycobacterium smegmatis protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.5 nm
Dmax 8.6 nm

SASDDT2 – EspG3 chaperone from Mycobacterium tuberculosis

EspG3 chaperone from Mycobacterium tuberculosis experimental SAS data
EspG3 chaperone from Mycobacterium tuberculosis Kratky plot
Sample: EspG3 chaperone from Mycobacterium tuberculosis , 34 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.5 nm
Dmax 9.0 nm

SASDDU2 – EspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled)

EspG3 chaperone from Mycobacterium smegmatis experimental SAS data
EspG3 chaperone from Mycobacterium smegmatis Kratky plot
Sample: EspG3 chaperone from Mycobacterium smegmatis monomer, 32 kDa Mycobacterium smegmatis protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.6 nm
Dmax 9.2 nm

SASDDV2 – EspG5 chaperone from Mycobacterium tuberculosis

EspG5 chaperone from Mycobacterium tuberculosis experimental SAS data
EspG5 chaperone from Mycobacterium tuberculosis Rg histogram
Sample: EspG5 chaperone from Mycobacterium tuberculosis monomer, 32 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 12
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.4 nm
Dmax 8.0 nm

SASDDW2 – EspG5-PE25/PPE41 complex from M. tuberculosis

EspG5 chaperone from Mycobacterium tuberculosisPE25 from Mycobacterium tuberculosisPPE41 from Mycobacterium tuberculosis experimental SAS data
Sample: EspG5 chaperone from Mycobacterium tuberculosis monomer, 32 kDa Mycobacterium tuberculosis protein
PE25 from Mycobacterium tuberculosis monomer, 11 kDa Mycobacterium tuberculosis protein
PPE41 from Mycobacterium tuberculosis monomer, 22 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Feb 10
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 4.0 nm
Dmax 13.0 nm

SASDDX2 – EspG3-PE5/PPE4 complex from M. tuberculosis

EspG3 chaperone from Mycobacterium tuberculosisPE5 from Mycobacterium tuberculosisPPE4 from Mycobacterium tuberculosis experimental SAS data
Sample: EspG3 chaperone from Mycobacterium tuberculosis , 34 kDa Mycobacterium tuberculosis protein
PE5 from Mycobacterium tuberculosis monomer, 10 kDa Mycobacterium tuberculosis protein
PPE4 from Mycobacterium tuberculosis monomer, 52 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Jun 15
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 4.0 nm
Dmax 14.2 nm

SASDD33 – Toxin/Antitoxin complex from M. tuberculosis

Mycobacterial cidal toxinMycobacterial cidal antitoxin experimental SAS data
PDB model
Sample: Mycobacterial cidal toxin hexamer, 121 kDa Mycobacterium tuberculosis protein
Mycobacterial cidal antitoxin hexamer, 76 kDa Mycobacterium tuberculosis protein
Buffer: 100 mM HEPES, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death. Mol Cell (2019)
...Tuukkanen A, Boudehen YM, Peixoto A, Botella L, Svergun DI, Schnappinger D, Schneider TR, Genevaux P, de Carvalho LPS, Wilmanns M, Parret AHA, Neyrolles O
RgGuinier 4.1 nm
Dmax 11.4 nm
VolumePorod 262 nm3

SASDFC6 – Wild type protein kinase YopO

Protein kinase YopO experimental SAS data
CUSTOM IN-HOUSE model
Sample: Protein kinase YopO monomer, 63 kDa Yersinia enterocolitica protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 6
Studying Conformational Changes of the Yersinia Type-III-Secretion Effector YopO in Solution by Integrative Structural Biology. Structure 27(9):1416-1426.e3 (2019)
...Tuukkanen AT, Heubach CA, Selsam A, Duthie FG, Svergun DI, Schiemann O, Hagelueken G
RgGuinier 3.3 nm
Dmax 11.5 nm
VolumePorod 119 nm3

SASDFD6 – Protein kinase YopO - cytoplasmic 1 actin complex

Protein kinase YopOActin, cytoplasmic 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Protein kinase YopO monomer, 63 kDa Yersinia enterocolitica protein
Actin, cytoplasmic 1 monomer, 42 kDa Homo sapiens protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 6
Studying Conformational Changes of the Yersinia Type-III-Secretion Effector YopO in Solution by Integrative Structural Biology. Structure 27(9):1416-1426.e3 (2019)
...Tuukkanen AT, Heubach CA, Selsam A, Duthie FG, Svergun DI, Schiemann O, Hagelueken G
RgGuinier 3.6 nm
Dmax 12.3 nm
VolumePorod 147 nm3

SASDAH6 – WbdD(1-459)

bifunctional kinase- methyltransferase WbdD experimental SAS data
CORAL model
Sample: bifunctional kinase- methyltransferase WbdD monomer, 59 kDa Escherichia coli protein
Buffer: 20 mM BisTris 50 mM NaCl 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Sep 23
A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide. Nat Struct Mol Biol 22(1):50-56 (2015)
...Tuukkanen A, Danciu I, Svergun DI, Hussain R, Liu H, Whitfield C, Naismith JH
RgGuinier 3.1 nm
Dmax 10.0 nm
VolumePorod 90 nm3

SASDAJ6 – WbdD(1-556)

bifunctional kinase- methyltransferase WbdD experimental SAS data
CORAL model
Sample: bifunctional kinase- methyltransferase WbdD trimer, 190 kDa protein
Buffer: 20 mM BisTris 50 mM NaCl 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Dec 2
A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide. Nat Struct Mol Biol 22(1):50-56 (2015)
...Tuukkanen A, Danciu I, Svergun DI, Hussain R, Liu H, Whitfield C, Naismith JH
RgGuinier 5.2 nm
Dmax 17.0 nm
VolumePorod 380 nm3

SASDAL6 – Wild-type chalcone isomerase, ligand-free

Bacterial chalcone isomerase experimental SAS data
Bacterial chalcone isomerase Kratky plot
Sample: Bacterial chalcone isomerase hexamer, 194 kDa Eubacterium ramulus protein
Buffer: 50 mM sodium phosphate, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase. Acta Crystallogr D Biol Crystallogr 71(Pt 4):907-17 (2015)
...Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W
RgGuinier 4.0 nm
Dmax 13.0 nm
VolumePorod 320 nm3

SASDAM6 – Naringenin-bound chalcone isomerase

Chalcone isomerase with Naringenin experimental SAS data
Chalcone isomerase with Naringenin Kratky plot
Sample: Chalcone isomerase with Naringenin hexamer, 194 kDa Eubacterium ramulus protein
Buffer: 50 mM sodium phosphate, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase. Acta Crystallogr D Biol Crystallogr 71(Pt 4):907-17 (2015)
...Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W
RgGuinier 3.7 nm
Dmax 11.0 nm
VolumePorod 320 nm3

SASDAN6 – Chalcone isomerase, CHI_Δlid construct

Chalcone isomerase deltaLid experimental SAS data
Chalcone isomerase deltaLid Kratky plot
Sample: Chalcone isomerase deltaLid hexamer, 181 kDa Eubacterium ramulus protein
Buffer: 50 mM sodium phosphate, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase. Acta Crystallogr D Biol Crystallogr 71(Pt 4):907-17 (2015)
...Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W
RgGuinier 3.6 nm
Dmax 11.0 nm
VolumePorod 270 nm3

SASDC97 – Ethylene Receptor 1 (DHp + CA + RD domains)

Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains experimental SAS data
Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains Kratky plot
Sample: Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains dimer, 88 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Oct 11
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Tuukkanen A, Mertens HD, Mueller-Dieckmann J
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 144 nm3

SASDCA7 – Ethylene Receptor 1 (DHp + CA domains)

Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains experimental SAS data
Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains Kratky plot
Sample: Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains dimer, 55 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Oct 11
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Tuukkanen A, Mertens HD, Mueller-Dieckmann J
RgGuinier 2.7 nm
Dmax 8.7 nm
VolumePorod 74 nm3

SASDEJ7 – The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex

Surface presentation of antigens protein SpaO SpaO(SPOA2)Surface presentation of antigens protein SpaO(SPOA1,2)Oxygen-regulated invasion protein OrgBATP synthase  InvC experimental SAS data
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Oxygen-regulated invasion protein OrgB ATP synthase  InvC Kratky plot
Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein
Surface presentation of antigens protein SpaO(SPOA1,2) monomer, 34 kDa Salmonella enterica subsp. … protein
Oxygen-regulated invasion protein OrgB dimer, 53 kDa Salmonella enterica subsp. … protein
ATP synthase InvC monomer, 48 kDa Salmonella enterica subsp. … protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 12
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
...Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 6.0 nm
Dmax 22.7 nm
VolumePorod 302 nm3

SASDEK7 – SpaO C-terminus

Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus experimental SAS data
Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus Kratky plot
Sample: Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus monomer, 19 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
...Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 2.1 nm
Dmax 7.2 nm

SASDA28 – anti-TG2 antibody (679 14 E06)

anti-TG2 antibody (679 14 E06)  experimental SAS data
CRYSOL model
Sample: anti-TG2 antibody (679 14 E06) monomer, 48 kDa protein
Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17
Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease. J Biol Chem 290(35):21365-75 (2015)
...Tuukkanen A, Svergun D, Sollid LM
RgGuinier 2.5 nm
Dmax 8.1 nm
VolumePorod 58 nm3

SASDA38 – transglutaminase-2 (TGA2)

transglutaminase 2 experimental SAS data
CRYSOL model
Sample: transglutaminase 2 monomer, 79 kDa Homo sapiens protein
Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17
Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease. J Biol Chem 290(35):21365-75 (2015)
...Tuukkanen A, Svergun D, Sollid LM
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 117 nm3

SASDA48 – transglutaminase2:anti-transglutaminase2 FAB1 antibody complex

anti-TG2 antibody (679 14 E06) transglutaminase 2 experimental SAS data
DAMMIN model
Sample: anti-TG2 antibody (679 14 E06) monomer, 48 kDa protein
transglutaminase 2 monomer, 79 kDa Homo sapiens protein
Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17
Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease. J Biol Chem 290(35):21365-75 (2015)
...Tuukkanen A, Svergun D, Sollid LM
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 168 nm3

SASDC68 – Surface presentation of antigens protein, SpaOc

Surface presentation of antigens protein SpaO SpaO(SPOA2) experimental SAS data
Surface presentation of antigens protein SpaO SpaO(SPOA2) Kratky plot
Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
...Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 2.5 nm
Dmax 9.1 nm
VolumePorod 51 nm3

SASDC78 – Surface presentation of antigens protein SpaOc-SpaO(SPOA1,2)

Surface presentation of antigens protein SpaO SpaO(SPOA2)Surface presentation of antigens protein SpaO(SPOA1,2) experimental SAS data
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Kratky plot
Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein
Surface presentation of antigens protein SpaO(SPOA1,2) monomer, 34 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
...Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 3.3 nm
Dmax 11.1 nm
VolumePorod 108 nm3

SASDC88 – Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus

Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus experimental SAS data
Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus Kratky plot
Sample: Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus monomer, 17 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
...Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 1.6 nm
Dmax 5.2 nm
VolumePorod 31 nm3

SASDC98 – Surface presentation of antigens protein SpaOc-SpaO N-terminus

Surface presentation of antigens protein SpaO SpaO(SPOA2)Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus experimental SAS data
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus Kratky plot
Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein
Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus monomer, 17 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
...Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 2.9 nm
Dmax 10.6 nm
VolumePorod 68 nm3

SASDEP9 – Cyclic GMP-AMP synthase (cGAS)

Cyclic GMP-AMP synthase experimental SAS data
Cyclic GMP-AMP synthase (cGAS) Rg histogram
Sample: Cyclic GMP-AMP synthase monomer, 61 kDa Homo sapiens protein
Buffer: 20 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 25
cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity. EMBO Rep (2019)
...Tuukkanen A, Kolbe M, Dorhoi A, Kaufmann SH
RgGuinier 3.1 nm
Dmax 12.7 nm
VolumePorod 110 nm3

SASDEQ9 – Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP)

Cyclic GMP-AMP synthase2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine experimental SAS data
Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP) Rg histogram
Sample: Cyclic GMP-AMP synthase dimer, 123 kDa Homo sapiens protein
2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine dimer, 1 kDa
Buffer: 20 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 25
cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity. EMBO Rep (2019)
...Tuukkanen A, Kolbe M, Dorhoi A, Kaufmann SH
RgGuinier 3.9 nm
Dmax 14.1 nm
VolumePorod 127 nm3