A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions.

Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
ACS Comb Sci 20(4):197-202 (2018 Apr 9)

PMID: 29553252 doi: 10.1021/acscombsci.8b00007

Submitted to SASBDB: 2018 Mar 15 Published in SASBDB: 2018 Mar 21

SASDDX4 – Wild-type Protein sex-lethal Sample: Protein sex-lethal monomer, 20 kDa Drosophila melanogaster protein Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 16 RgGuinier 2.0 nm Dmax 6.9 nm VolumePorod 26 nm3

SASDDY4 – Protein sex-lethal mutant with 10GS-linker Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6 Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 2 RgGuinier 2.1 nm Dmax 7.4 nm VolumePorod 27 nm3

SASDDZ4 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein RNA decaneucleotide U8GU monomer, 3 kDa synthetic construct RNA Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6 Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 3 RgGuinier 2.2 nm Dmax 7.8 nm VolumePorod 34 nm3

SASDD25 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8 Sample: Protein sex-lethal mutant dimer, 41 kDa Drosophila melanogaster protein RNA decaneucleotide UGU8 dimer, 6 kDa synthetic construct RNA Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 12 RgGuinier 2.5 nm Dmax 8.9 nm VolumePorod 41 nm3