Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes.

Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
Sci Rep 9(1):2465 (2019 Feb 21)

PMID: 30792490 doi: 10.1038/s41598-019-39213-2

Submitted to SASBDB: 2018 Mar 29 Published in SASBDB: 2019 Feb 25

SASDDG5 – Mammalian prion protein mRNA (PrP mRNA wild type) Sample: Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA Buffer: 10 mM Tris buffer, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Nov 4 RgGuinier 7.4 nm Dmax 25.0 nm VolumePorod 165 nm3

SASDDH5 – Mammalian prion protein mRNA (PrP mRNA wild type) with KCl Sample: Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA Buffer: 10 mM Tris buffer with 100 mM KCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6 RgGuinier 8.8 nm Dmax 31.0 nm VolumePorod 240 nm3

SASDDJ5 – Mammalian prion protein mRNA (PrP mRNA wild type) with LiCl Sample: Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA Buffer: 10 mM Tris buffer with 100 mM LiCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6 RgGuinier 9.0 nm Dmax 31.0 nm VolumePorod 225 nm3

SASDDK5 – Mammalian prion protein mRNA (PrP mRNA wild type) with KCl and pyridostatin (PDS) Sample: Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA Buffer: 10 mM Tris buffer with 100 mM KCl and 1 mM PDS, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6 RgGuinier 9.1 nm Dmax 31.0 nm VolumePorod 220 nm3

SASDDL5 – Mutant mammalian prion protein mRNA (octo-repeat PrP mRNA) Sample: Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA Buffer: 10 mM Tris buffer, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6 RgGuinier 7.0 nm Dmax 24.0 nm VolumePorod 150 nm3

SASDDM5 – Mutant mammalian prion protein mRNA (octo-repeat PrP mRNA) with KCl Sample: Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA Buffer: 10 mM Tris buffer with 100 mM KCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6 RgGuinier 9.0 nm Dmax 33.0 nm VolumePorod 210 nm3

SASDDN5 – Mutant mammalian prion protein mRNA (octo-repear PrP mRNA) with LiCl Sample: Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA Buffer: 10 mM Tris buffer with 100 mM LiCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6 RgGuinier 8.9 nm Dmax 33.0 nm VolumePorod 223 nm3

SASDDP5 – Mutant mammalian prion protein mRNA (octo-repear PrP mRNA) with KCl and pyridostatin (PDS) Sample: Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA Buffer: 10 mM Tris buffer with 100 mM KCl and 1 mM PDS, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6 RgGuinier 9.2 nm Dmax 33.0 nm VolumePorod 260 nm3