Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks.

Kobayashi N, Inano K, Sasahara K, Sato T, Miyazawa K, Fukuma T, Hecht MH, Song C, Murata K, Arai R
ACS Synth Biol 7(5):1381-1394 (2018 May 18)

PMID: 29690759 doi: 10.1021/acssynbio.8b00007

Submitted to SASBDB: 2018 Apr 12 Published in SASBDB: 2018 May 7

SASDD46 – Artificially designed de novo protein esPN-Block (HL4) heterocomplex, e1s2 (HL4) Sample: Extender PN-Block (HL4) monomer, 27 kDa de novo protein protein Stopper PN-Block (WA20) dimer, 25 kDa de novo protein protein Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2014 Dec 19 RgGuinier 3.6 nm Dmax 15.0 nm

SASDD56 – Artificially designed de novo protein esPN-Block (FL4) heterocomplex, e1s2 (FL4) Sample: Stopper PN-Block (WA20) dimer, 25 kDa de novo protein protein Extender PN-Block (FL4) monomer, 27 kDa de novo protein protein Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2014 Dec 19 RgGuinier 3.3 nm Dmax 12.0 nm