Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states.

Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
IUCrJ 5(Pt 6):780-793 (2018 Nov 1)

PMID: 30443361 doi: 10.1107/S2052252518012186

Submitted to SASBDB: 2018 Apr 10 Published in SASBDB: 2018 Oct 10

SASDDY5 – AMPA subtype ionotropic Glutamate receptor GluA2 in the resting state (apo), in stealth DDM detergents Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein Buffer: D2O based buffer. 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5 Experiment: SANS data collected at KWS1, FRM2 on 2017 Sep 19 RgGuinier 6.0 nm Dmax 17.9 nm VolumePorod 396 nm3

SASDDZ5 – AMPA subtype ionotropic Glutamate receptor GluA2 in the AMPA bound state, in stealth DDM detergents, pH 7.5 Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein Buffer: D2O based buffer. 1 mM AMPA, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5 Experiment: SANS data collected at KWS1, FRM2 on 2016 Oct 19 RgGuinier 6.3 nm Dmax 18.4 nm VolumePorod 407 nm3

SASDD26 – AMPA subtype ionotropic Glutamate receptor GluA2 in the AMPA bound state, in stealth DDM detergents, pH 5.5 Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein Buffer: D2O based buffer. 10 mM AMPA, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 5.5 Experiment: SANS data collected at KWS1, FRM2 on 2017 Sep 19 RgGuinier 6.5 nm Dmax 18.9 nm VolumePorod 899 nm3

SASDD36 – AMPA subtype ionotropic Glutamate receptor GluA2 in the GYKI-53655 bound state, in stealth DDM detergents Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein Buffer: D2O based buffer. 1 mM GYKI-53655, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5 Experiment: SANS data collected at KWS1, FRM2 on 2016 Oct 19 RgGuinier 6.3 nm Dmax 18.6 nm VolumePorod 384 nm3