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21 hits found for Larsen

SASDM42 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
RgGuinier 4.2 nm
Dmax 13.9 nm

SASDM52 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles with bound Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
RgGuinier 4.3 nm
Dmax 13.9 nm

SASDM62 – Cobalt/magnesium transport protein CorA in matched-out deuterated nanodiscs without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
RgGuinier 4.8 nm
Dmax 14.2 nm

SASDM72 – Cobalt/magnesium transport protein CorA in match-out deuterated nanodiscs with bound Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
RgGuinier 4.9 nm
Dmax 14.3 nm

SASDEF2 – Mitochondrial import inner membrane translocase complex TIM9·10

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CORAL model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Tris, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Larsen K, Wiedemann N, Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDEG2 – Mitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial GTP/GDP carrier protein 1 experimental SAS data
DAMMIF model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 hexamer, 61 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 hexamer, 61 kDa Saccharomyces cerevisiae protein
Mitochondrial GTP/GDP carrier protein 1 monomer, 33 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Tris, 150mM NaCl, imidiazole, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Larsen K, Wiedemann N, Schanda P
RgGuinier 4.5 nm
Dmax 16.0 nm
VolumePorod 272 nm3

SASDKA4 – Complement factor P, properdin (FP) dimer

Properdin (dimer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (dimer) dimer, 110 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
...Larsen K, Arleth L, Andersen GR
RgGuinier 8.1 nm
Dmax 24.0 nm

SASDKB4 – Complement factor P, properdin (FP) trimer

Properdin (trimer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (trimer) trimer, 165 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
...Larsen K, Arleth L, Andersen GR
RgGuinier 10.2 nm
Dmax 27.0 nm

SASDKC4 – Complement factor P, properdin (FP) tetramer

Properdin (tetramer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (tetramer) tetramer, 220 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
...Larsen K, Arleth L, Andersen GR
RgGuinier 13.1 nm
Dmax 36.0 nm

SASDD35 – Nanodiscs with membrane scaffold protein 1D1 (MSP1D1) and dilaurylphosphatidyl choline (DLPC)

Membrane scaffold protein 1D1 experimental SAS data
Sample: Membrane scaffold protein 1D1 dimer, 50 kDa unidentified protein
Buffer: 20 mM Tris, 100 mM NaCl, 100 mM sodium cholate,, pH: 7.4
Experiment: SAXS data collected at ID14-3, ESRF on 2009 Nov 14
Elliptical structure of phospholipid bilayer nanodiscs encapsulated by scaffold proteins: casting the roles of the lipids and the protein. J Am Chem Soc 132(39):13713-22 (2010)
Skar-Gislinge N, Simonsen JB, Mortensen K, Feidenhans'l R, Sligar SG, Lindberg Møller B, Bjørnholm T, Arleth L
RgGuinier 4.9 nm
Dmax 12.2 nm

SASDDY5 – AMPA subtype ionotropic Glutamate receptor GluA2 in the resting state (apo), in stealth DDM detergents

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2017 Sep 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.0 nm
Dmax 17.9 nm
VolumePorod 396 nm3

SASDDZ5 – AMPA subtype ionotropic Glutamate receptor GluA2 in the AMPA bound state, in stealth DDM detergents, pH 7.5

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 1 mM AMPA, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2016 Oct 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.3 nm
Dmax 18.4 nm
VolumePorod 407 nm3

SASDD26 – AMPA subtype ionotropic Glutamate receptor GluA2 in the AMPA bound state, in stealth DDM detergents, pH 5.5

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 10 mM AMPA, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 5.5
Experiment: SANS data collected at KWS1, FRM2 on 2017 Sep 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.5 nm
Dmax 18.9 nm
VolumePorod 899 nm3

SASDD36 – AMPA subtype ionotropic Glutamate receptor GluA2 in the GYKI-53655 bound state, in stealth DDM detergents

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 1 mM GYKI-53655, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2016 Oct 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.3 nm
Dmax 18.6 nm
VolumePorod 384 nm3

SASDGC6 – Glucuronoyl esterase, deglycolsylated full length

4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase) experimental SAS data
DAMMIN model
Sample: 4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase) monomer, 51 kDa Cerrena unicolor protein
Buffer: 20 mM sodium acetate, pH: 5
Experiment: SAXS data collected at Xenocs BioXolver L with GeniX3D, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Oct 10
The structural basis of fungal glucuronoyl esterase activity on natural substrates. Nat Commun 11(1):1026 (2020)
...Larsen S
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 71 nm3

SASDGD6 – Glucuronoyl esterase S286A, deglycolsylated truncated (amino acids 95-474)

4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase, truncated) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase, truncated) monomer, 43 kDa Cerrena unicolor protein
Buffer: 20 mM sodium acetate, pH: 5
Experiment: SAXS data collected at Xenocs BioXolver L with GeniX3D, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Oct 10
The structural basis of fungal glucuronoyl esterase activity on natural substrates. Nat Commun 11(1):1026 (2020)
...Larsen S
RgGuinier 2.0 nm
Dmax 6.1 nm
VolumePorod 50 nm3

SASDQP6 – Nanodisc with POPC and circularized membrane scaffolding protein (csMSP1E3D1)

circularized Membrane scaffolding protein 1 E3 D11-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: circularized Membrane scaffolding protein 1 E3 D1 , 62 kDa protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) None, lipid
Buffer: 20 mM Tris-HCl pH 7.5, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5
Circularized and solubility‐enhanced MSP s facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution The FEBS Journal 286(9):1734-1751 (2019)
Johansen N, Tidemand F, Nguyen T, Rand K, Pedersen M, Arleth L
RgGuinier 5.8 nm
Dmax 14.5 nm

SASDH28 – Mitochondrial import inner membrane translocase TIM 9·10·12

Mitochondrial import inner membrane translocase subunit TIM12Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM12 monomer, 12 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM9 dimer, 20 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM MES buffer (2-(N-morpholino)ethanesulfonic acid), pH 6.5, 50 mM NaCl Cloning,, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2017 May 8
Architecture and subunit dynamics of the mitochondrial TIM9·10·12 chaperone (2020)
...Larsen K, Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDH89 – Mitochondrial TIM8·13 chaperone in complex with the Tim23 membrane protein precursor

Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13Mitochondrial import inner membrane translocase subunit TIM23 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 24
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Larsen K, Bersch B, Schanda P
RgGuinier 3.1 nm
Dmax 11.3 nm

SASDJP4 – Mitochondrial import inner membrane translocase subunits TIM9-TIM10 in complex with TIM23

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial import inner membrane translocase subunit TIM23 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 May 18
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Larsen K, Bersch B, Schanda P
RgGuinier 3.2 nm
Dmax 7.7 nm
VolumePorod 146 nm3

SASDJQ4 – Mitochondrial import inner membrane translocase subunits TIM8-TIM13

Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Larsen K, Bersch B, Schanda P
RgGuinier 3.1 nm
Dmax 10.9 nm
VolumePorod 136 nm3