Integrin α6β4 recognition of a linear motif of bullous pemphigoid antigen BP230 controls its recruitment to hemidesmosomes: Supplemental figures S1 to S10 and table S1

Manso J, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
(2018 Aug 31)

doi: 10.1101/402123
Submitted to SASBDB: 2018 Jun 7
Published in SASBDB:

SASDDE8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4)

Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4) Rg histogram
Sample: Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 37 nm3

SASDDF8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463E

Integrin beta-4 (1436-1666) R1463E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1463E monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDDG8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A

Integrin beta-4 (1436-1666) R1463A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1463A monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 36 nm3

SASDDH8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468R

Integrin beta-4 (1436-1666) A1468R experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468R Rg histogram
Sample: Integrin beta-4 (1436-1666) A1468R monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.8 nm
Dmax 10.9 nm
VolumePorod 35 nm3

SASDDJ8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468D

Integrin beta-4 (1436-1666) A1468D experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468D Rg histogram
Sample: Integrin beta-4 (1436-1666) A1468D monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 34 nm3

SASDDK8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475E

Integrin beta-4 (1436-1666) R1475E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1475E monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.6 nm
Dmax 9.5 nm
VolumePorod 42 nm3

SASDDL8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475A

Integrin beta-4 (1436-1666) R1475A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1475A monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDDM8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542E

Integrin beta-4 (1436-1666) R1542E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1542E monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDDN8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542A

Integrin beta-4 (1436-1666) R1542A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1542A monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.3 nm
Dmax 8.5 nm
VolumePorod 34 nm3

SASDDP8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant T1663R

Integrin beta-4 (1436-1666) T1663R experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant T1663R Rg histogram
Sample: Integrin beta-4 (1436-1666) T1663R monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 35 nm3