Human MICAL1: activation by the small GTPase Rab8 and small-angle X-ray scattering studies on the oligomerization state of MICAL1 and its complex with Rab8.

Esposito A, Ventura V, Petoukhov MV, Rai A, Svergun DI, Vanoni MA
Protein Sci (2018 Sep 22)

PMID: 30242933 doi: 10.1002/pro.3512

Submitted to SASBDB: 2018 Jul 17 Published in SASBDB: 2018 Sep 25

SASDDR9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) Sample: [F-actin]-monooxygenase MICAL1 (monomer) monomer, 118 kDa Homo sapiens protein Buffer: 50 mM sodium phosphate buffer, pH 7.5, 5 % glycerol, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 20 RgGuinier 3.7 nm Dmax 12.1 nm VolumePorod 212 nm3

SASDDS9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) (Truncated MOCHLIM construct) Sample: [F-actin]-monooxygenase MICAL1 (MoChLim) monomer, 85 kDa Homo sapiens protein Buffer: 20 mM Hepes/NaOH, pH 7.5, 50 mM NaCl, 2 mM MgCl2, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 20 RgGuinier 4.0 nm Dmax 18.0 nm VolumePorod 145 nm3

SASDDT9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) in complex with Ras-related protein Rab 8 (MICAL1-Rab8 complex) Sample: [F-actin]-monooxygenase MICAL1 (monomer) monomer, 118 kDa Homo sapiens protein Ras-related protein 8 monomer, 20 kDa protein Buffer: 20 mM Hepes/NaOH, pH 7.5, 50 mM NaCl, 2 mM MgCl2, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 20 RgGuinier 3.7 nm Dmax 11.8 nm VolumePorod 234 nm3

SASDDU9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) (Truncated MOCH construct) Sample: [F-actin]-monooxygenase MICAL1 (MoCh) monomer, 67 kDa Homo sapiens protein Buffer: 50 mM sodium phosphate buffer, 5 % glycerol, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 6 RgGuinier 3.4 nm Dmax 12.0 nm VolumePorod 100 nm3