Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain

Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
Biophysical Journal (2020 Jan)

doi: 10.1016/j.bpj.2019.12.025 		Submitted to SASBDB: 2018 Nov 13
Published in SASBDB:

SASDEW7 – C-terminal truncated bank vole prion protein (amino acids 90-231)

Major prion protein experimental SAS data
C-terminal truncated bank vole prion protein (amino acids 90-231) Rg histogram
Sample: Major prion protein monomer, 16 kDa Myodes glareolus protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 25
RgGuinier 2.3 nm
Dmax 9.4 nm
VolumePorod 29 nm3

SASDEX7 – C-terminal truncated bank vole prion protein (amino acids 90-231) bound to Cu(II)

Major prion protein experimental SAS data
C-terminal truncated bank vole prion protein (amino acids 90-231) bound to Cu(II) Rg histogram
Sample: Major prion protein monomer, 16 kDa Myodes glareolus protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 25
RgGuinier 2.2 nm
Dmax 8.7 nm
VolumePorod 30 nm3

SASDEY7 – C-terminal truncated ovine prion protein ARR (amino acids 94-234)

Major prion protein experimental SAS data
C-terminal truncated ovine prion protein ARR (amino acids 94-234) Rg histogram
Sample: Major prion protein monomer, 16 kDa Ovis aries protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 4
RgGuinier 2.2 nm
Dmax 9.0 nm
VolumePorod 29 nm3

SASDEZ7 – C-terminal truncated ovine prion protein (94-234) with ARR polymorphism + Cu(II)

Major prion protein experimental SAS data
C-terminal truncated ovine prion protein (94-234) with ARR polymorphism + Cu(II) Rg histogram
Sample: Major prion protein monomer, 16 kDa Ovis aries protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 4
RgGuinier 2.2 nm
Dmax 8.8 nm
VolumePorod 31 nm3

SASDE28 – C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism

Major prion protein experimental SAS data
C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism Rg histogram
Sample: Major prion protein monomer, 16 kDa Ovis aries protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 4
RgGuinier 2.1 nm
Dmax 9.1 nm
VolumePorod 29 nm3

SASDE38 – C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism + Cu(II)

Major prion protein experimental SAS data
C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism + Cu(II) Rg histogram
Sample: Major prion protein monomer, 16 kDa Ovis aries protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 4
RgGuinier 2.2 nm
Dmax 8.7 nm
VolumePorod 29 nm3