Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM.

Tosi T, Hoshiga F, Millership C, Singh R, Eldrid C, Patin D, Mengin-Lecreulx D, Thalassinos K, Freemont P, Gr√ľndling A
PLoS Pathog 15(1):e1007537 (2019 Jan 22)
PMID: 30668586
doi: 10.1371/journal.ppat.1007537
Submitted to SASBDB: 2018 Nov 17
Published in SASBDB:

SASDE78 – Solution structure of the diadenylate cyclase/phosphoglucosamine mutase (DacA/GlmM) complex from Staphylococcus aureus

Diadenylate cyclasePhosphoglucosamine mutase experimental SAS data
DAMFILT model
Sample: Diadenylate cyclase dimer, 39 kDa Staphylococcus aureus (strain ... protein
Phosphoglucosamine mutase dimer, 99 kDa Staphylococcus aureus (strain ... protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 7
RgGuinier 3.9 nm
Dmax 12.1 nm
VolumePorod 204 nm3

SASDE88 – Solution structure of phosphoglucosamine mutase (GlmM) from Staphylococcus aureus

Phosphoglucosamine mutase experimental SAS data
DAMFILT model
Sample: Phosphoglucosamine mutase dimer, 99 kDa Staphylococcus aureus (strain ... protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 7
RgGuinier 3.7 nm
Dmax 12.5 nm
VolumePorod 134 nm3

SASDE98 – Solution structure of Diadenylate cyclase (DacA) from Staphylococcus aureus

Diadenylate cyclase experimental SAS data
DAMFILT model
Sample: Diadenylate cyclase dimer, 39 kDa Staphylococcus aureus (strain ... protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 7
RgGuinier 2.6 nm
Dmax 8.6 nm
VolumePorod 57 nm3