Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless.

Ankush Jagtap PK, Müller M, Masiewicz P, von Bülow S, Hollmann NM, Chen PC, Simon B, Thomae AW, Becker PB, Hennig J
Nucleic Acids Res 47(8):4319-4333 (2019 May 7)

PMID: 30805612 doi: 10.1093/nar/gkz125

Submitted to SASBDB: 2019 Jan 28 Published in SASBDB: 2019 Feb 21

SASDF52 – dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE Sample: Dosage compensation regulator monomer, 29 kDa Drosophila melanogaster protein Buffer: 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 29 RgGuinier 3.2 nm Dmax 12.5 nm VolumePorod 22 nm3

SASDF62 – Mixture of the dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE, and the roX2 RNA stem-loop 7 18mer-fragment, at 1.0 mg/ml Sample: Dosage compensation regulator monomer, 29 kDa Drosophila melanogaster protein RoX2 stem-loop 7, 18-mer fragment monomer, 12 kDa synthetic construct RNA Buffer: 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 29 RgGuinier 3.1 nm Dmax 13.3 nm VolumePorod 25 nm3

SASDF72 – roX2 RNA stem-loop 7 18mer-fragment Sample: RoX2 stem-loop 7, 18-mer fragment monomer, 12 kDa synthetic construct RNA Buffer: 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 29 RgGuinier 1.8 nm Dmax 8.5 nm VolumePorod 14 nm3