How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids.

Mias-Lucquin D, Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
J Struct Biol :107411 (2019 Nov 2)

PMID: 31689503 doi: 10.1016/j.jsb.2019.107411

Submitted to SASBDB: 2019 Mar 30 Published in SASBDB: 2019 Nov 14

SASDFW4 – Conformation of R8-15 human dystrophin fragment Sample: Human dystrophin central domain R8-15 fragment monomer, 100 kDa protein Buffer: NaP 10 mM, NaCl 500 mM, EDTA 1 mM, Glycerol 2%, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Sep 23 RgGuinier 10.1 nm Dmax 36.0 nm

SASDFX4 – Conformation of R11-19 human dystrophin fragment Sample: Human dystrophin central domain R11-19 fragment monomer, 117 kDa protein Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycerol 2%, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2017 May 11 RgGuinier 8.8 nm Dmax 37.5 nm VolumePorod 513 nm3

SASDFT4 – Conformation of the R11-15 human dystrophin fragment (SANS) Sample: Dystrophin (R11-15 human dystrophin fragment) monomer, 60 kDa Homo sapiens protein Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% v/v D2O, pH: 7.1 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 1 RgGuinier 6.2 nm Dmax 27.4 nm VolumePorod 146 nm3

SASDFU4 – Conformation of R11-15 human dystrophin fragment in interaction with zwitterionic phospholipid bicelles (SANS) Sample: Dystrophin (R11-15 human dystrophin fragment) monomer, 60 kDa Homo sapiens protein Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% v/v D2O, pH: 7.1 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 1 RgGuinier 6.2 nm Dmax 28.1 nm VolumePorod 144 nm3

SASDFV4 – Conformation of R11-15 human dystrophin fragment in interaction with anionic phospholipid bicelles (SANS) Sample: Dystrophin (R11-15 human dystrophin fragment) monomer, 60 kDa Homo sapiens protein Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% v/v D2O, pH: 7.1 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 1 RgGuinier 8.1 nm Dmax 29.6 nm VolumePorod 231 nm3