Conformationally constrained mutant of human matrix metalloproteinase-1

Rob Holland

Submitted to SASBDB: 2019 Mar 26 Published in SASBDB: 2021 Sep 27

SASDFM4 – Matrix metalloproteinase-1 (MMP-1) Sample: Matrix metalloproteinase-1 (Interstitial collagenase) monomer, 43 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2016 May 14 RgGuinier 2.6 nm Dmax 8.3 nm VolumePorod 50 nm3

SASDFN4 – Domain-stapled mutant of Matrix metalloproteinase-1 (MMP-1 S243C, S318C) Sample: Matrix metalloproteinase-1 (Interstitial collagenase) (S243C, S318C) monomer, 43 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2016 May 14 RgGuinier 2.6 nm Dmax 7.7 nm VolumePorod 43 nm3

SASDFP4 – Pro-matrix metalloproteinase-1 (MMP-1) Sample: Pro-matrix metalloproteinase-1 (Interstitial collagenase) monomer, 52 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2015 May 21 RgGuinier 2.7 nm Dmax 9.0 nm VolumePorod 73 nm3

SASDFQ4 – Domain-stapled mutant of Pro-Matrix metalloproteinase-1 (proMMP-1 S243C, S318C) Sample: Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C) monomer, 52 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2015 May 21 RgGuinier 2.7 nm Dmax 8.9 nm VolumePorod 61 nm3