Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization

Sharma M, Aswal V, Kumar V, Chidambaram R
IUCrJ 7(2) (2020 Jan 25)

doi: 10.1107/S2052252519017159

Submitted to SASBDB: 2019 Jun 9 Published in SASBDB: 2020 Feb 3

SASDF87 – Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O Sample: Binary toxin receptor Cqm1 protein dimer, 129 kDa synthetic construct protein Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5 Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24 RgGuinier 2.8 nm Dmax 9.7 nm

SASDF97 – Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O Sample: Binary toxin receptor Cqm1 protein monomer, 65 kDa synthetic construct protein Deuterated BinB component of mosquito-larvicidal Binary toxin monomer, 56 kDa synthetic construct protein Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5 Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 23 RgGuinier 1.9 nm Dmax 7.3 nm

SASDFA7 – Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O Sample: Lysinibacillus Mosquito-larvicidal receptor binding component monomer, 53 kDa synthetic construct protein Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5 Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24 RgGuinier 3.3 nm Dmax 9.9 nm