Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.

Chen X, Hnida K, Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM
J Biol Chem 290(35):21365-75 (2015 Aug 28)

PMID: 26160175 doi: 10.1074/jbc.M115.669895

Submitted to SASBDB: 2015 Apr 14 Published in SASBDB: 2015 Aug 28

SASDA28 – anti-TG2 antibody (679 14 E06) Sample: Anti-TG2 antibody (679 14 E06) monomer, 48 kDa protein Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17 RgGuinier 2.5 nm Dmax 8.1 nm VolumePorod 58 nm3

SASDA38 – transglutaminase-2 (TGA2) Sample: Transglutaminase 2 monomer, 79 kDa Homo sapiens protein Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17 RgGuinier 3.4 nm Dmax 12.0 nm VolumePorod 117 nm3

SASDA48 – transglutaminase2:anti-transglutaminase2 FAB1 antibody complex Sample: Anti-TG2 antibody (679 14 E06) monomer, 48 kDa protein Transglutaminase 2 monomer, 79 kDa Homo sapiens protein Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17 RgGuinier 4.0 nm Dmax 13.9 nm VolumePorod 168 nm3