Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase.

Marsin S, Adam Y, Cargemel C, Andreani J, Baconnais S, Legrand P, Li de la Sierra-Gallay I, Humbert A, Aumont-Nicaise M, Velours C, Ochsenbein F, Durand D, Le Cam E, Walbott H, Possoz C, Quevillon-Cheruel S, Ferat JL
Nucleic Acids Res (2021 Jun 9)

PMID: 34107018 doi: 10.1093/nar/gkab463

Submitted to SASBDB: 2019 Sep 19 Published in SASBDB: 2021 May 24

SASDGP5 – Vibrio cholerae DciA Sample: DciA monomer, 18 kDa Vibrio cholerae serotype … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Mar 26 RgGuinier 2.7 nm Dmax 10.1 nm VolumePorod 26 nm3

SASDGQ5 – Vibrio cholerae hexameric DnaB helicase with bound ATP (VcDnaB.ATP) Sample: Replicative DNA helicase (DnaB) hexamer, 317 kDa Vibrio cholerae serotype … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH: 8.8 Experiment: SAXS data collected at SWING, SOLEIL on 2018 Oct 4 RgGuinier 4.6 nm Dmax 13.0 nm VolumePorod 585 nm3

SASDGR5 – The Vibrio cholerae DciA/DnaB helicase complex in the presence of bound ATP (VcDnaB.DciA.ATP) Sample: DnaB helicase complexed with ATP hexamer, 317 kDa Vibrio cholerae serotype … protein DciA, 55 kDa Vibrio cholerae serotype … protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH: 8.8 Experiment: SAXS data collected at SWING, SOLEIL on 2018 Oct 4 RgGuinier 5.0 nm Dmax 15.6 nm VolumePorod 670 nm3