Unfolding of monomeric lipoprotein lipase by ANGPTL4: Insight into the regulation of plasma triglyceride metabolism

Kristensen K, Leth-Espensen K, Mertens H, Birrane G, Meiyappan M, Olivecrona G, Jørgensen T, Young S, Ploug M
Proceedings of the National Academy of Sciences :201920202 (2020 Feb 07)

doi: 10.1073/pnas.1920202117
Submitted to SASBDB: 2019 Dec 16
Published in SASBDB:

SASDHF4 – Lipoprotein lipase-GPIHBP1-monoclonal antibody (5D2) complex

Lipoprotein lipaseGlycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1Monoclonal Antibody Fragment 5D2 experimental SAS data
CORAL model
Sample: Lipoprotein lipase monomer, 50 kDa Homo sapiens protein
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 monomer, 15 kDa Homo sapiens protein
Monoclonal Antibody Fragment 5D2 monomer, 47 kDa Mus musculus protein
Buffer: 10 mM Tris, 150 mM NaCl, 4 mM CaCL2, 10% (v/v) Glycerol, 0.05% 0.8mM CHAPS, ,0.05 % (v/v) NaN3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 14
RgGuinier 4.9 nm
Dmax 17.5 nm
VolumePorod 187 nm3