Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D

Kielkopf C, Whitten A, Garner B, Brown S
Bioscience Reports 41(1) (2021 Jan 05)

doi: 10.1042/BSR20201423

Submitted to SASBDB: 2020 Jan 20 Published in SASBDB: 2021 Mar 22

SASDHJ5 – Apolipoprotein-D plus biliverdin Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11 RgGuinier 3.3 nm Dmax 10.5 nm VolumePorod 168 nm3

SASDHK5 – Apolipoprotein-D oxidised with H2O2 Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11 RgGuinier 3.3 nm Dmax 10.3 nm VolumePorod 164 nm3

SASDHL5 – Apolipoprotein-D plus palmitic acid Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11 RgGuinier 3.3 nm Dmax 10.6 nm VolumePorod 163 nm3

SASDHM5 – Apolipoprotein-D plus progesterone Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11 RgGuinier 3.3 nm Dmax 10.0 nm VolumePorod 161 nm3

SASDHN5 – Apolipoprotein-D plus sphingomyelin Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11 RgGuinier 3.3 nm Dmax 9.9 nm VolumePorod 171 nm3