Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae.

Mazurkewich S, Helland R, Mackenzie A, Eijsink VGH, Pope PB, Brändén G, Larsbrink J
Sci Rep 10(1):13775 (2020 Aug 13)
PMID: 32792608
doi: 10.1038/s41598-020-70749-w
Submitted to SASBDB: 2020 Apr 14
Published in SASBDB:

SASDHU9 – Solution structure of middle domain of the multi-domain GH18 chitinase ChiA from Flavobacterium johnsoniae

Chitinase ChiA experimental SAS data
DAMMIN model
Sample: Chitinase ChiA monomer, 70 kDa Flavobacterium johnsoniae protein
Buffer: 50 mM Tris, 250 mM NaCl, 0.25 mM DTT, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 May 7
RgGuinier 4.7 nm
Dmax 16.3 nm
VolumePorod 99 nm3