Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach

Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L
Journal of Applied Crystallography 53(5) (2020 Sep 25)

doi: 10.1107/S1600576720010687

Submitted to SASBDB: 2020 Feb 15 Published in SASBDB: 2020 Sep 1

SASDHY5 – In cellulo luciferase protein crystals recombinantly expressed within High Five insect cells Sample: Photinus pyralis firefly luciferase, 61 kDa Photinus pyralis protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDJY5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells Sample: Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells Sample: Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDJZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells Sample: Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDH26 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells Sample: Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH36 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells Sample: Woronin body major protein, 19 kDa Neurospora crassa protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH46 – High Five insect cell culture infected with mock recombinant baculovirus (rBV) Sample: Mock recombinant baculovirus, 0 kDa unidentified baculovirus Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH56 – High Five insect cell culture (control; uninfected) Sample: High Five insect cells, 0 kDa Trichoplusia ni Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH66 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series) Sample: Woronin body major protein, 19 kDa Neurospora crassa protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH76 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series) Sample: Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH86 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells Sample: Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDH96 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within Sf9 insect cells Sample: Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHA6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells Sample: Woronin body major protein, 19 kDa Neurospora crassa protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHB6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells Sample: Woronin body major protein, 19 kDa Neurospora crassa protein Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21