Detection of in cellulo protein crystals using SAXS

Cy M Jeffries Submitted to SASBDB: 2020 Feb 15
Published in SASBDB:

SASDHY5 – In cellulo luciferase protein crystals recombinantly expressed within High Five insect cells

Photinus pyralis firefly luciferase experimental SAS data
Photinus pyralis firefly luciferase Kratky plot
Sample: Photinus pyralis firefly luciferase, 61 kDa Photinus pyralis protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDJY5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data
Inosine-5'-monophosphate dehydrogenase Kratky plot
Sample: Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data
Inosine-5'-monophosphate dehydrogenase Kratky plot
Sample: Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDJZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data
Inosine-5'-monophosphate dehydrogenase Kratky plot
Sample: Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDH26 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data
Cathepsin B-like cysteine protease Kratky plot
Sample: Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH36 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH46 – High Five insect cell culture infected with mock recombinant baculovirus (rBV)

mock recombinant baculovirus experimental SAS data
mock recombinant baculovirus Kratky plot
Sample: Mock recombinant baculovirus, 0 kDa unidentified baculovirus
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH56 – High Five insect cell culture (control; uninfected)

High Five insect cells experimental SAS data
High Five insect cells Kratky plot
Sample: High Five insect cells, 0 kDa Trichoplusia ni
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH66 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH76 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Cathepsin B-like cysteine protease experimental SAS data
Cathepsin B-like cysteine protease Kratky plot
Sample: Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH86 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data
Cathepsin B-like cysteine protease Kratky plot
Sample: Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDH96 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within Sf9 insect cells

Cathepsin B-like cysteine protease experimental SAS data
Cathepsin B-like cysteine protease Kratky plot
Sample: Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHA6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHB6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells

Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21