SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique

Stachowski T, Snell M, Snell E, Boggon T
PLOS ONE 15(11):e0239702 (2020 Nov 17)

doi: 10.1371/journal.pone.0239702

Submitted to SASBDB: 2020 Feb 24 Published in SASBDB: 2020 Nov 19

SASDHG6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 36.3 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 3.0 nm VolumePorod 69 nm3

SASDHH6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 72.6 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 3.1 nm VolumePorod 68 nm3

SASDHJ6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 108.9 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 3.0 nm VolumePorod 67 nm3

SASDHK6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 145.2 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 3.0 nm VolumePorod 66 nm3

SASDHL6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 181.5 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.9 nm VolumePorod 65 nm3

SASDHM6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 217.8 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.9 nm VolumePorod 64 nm3

SASDHN6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 254.1 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.9 nm VolumePorod 64 nm3

SASDHP6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 290.4 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.9 nm VolumePorod 63 nm3

SASDHQ6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 326.7 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 62 nm3

SASDHR6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 363 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 62 nm3

SASDHS6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 399.3 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.9 nm VolumePorod 62 nm3

SASDHT6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 435.6 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 63 nm3

SASDHU6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 471.9 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 60 nm3

SASDHV6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 508.2 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 61 nm3

SASDHW6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 544.5 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 63 nm3

SASDHX6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 580.8 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 61 nm3

SASDHY6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 617.1 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 60 nm3

SASDHZ6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 653.4 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 60 nm3

SASDH27 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 689.7 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 63 nm3

SASDH37 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 726 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 61 nm3

SASDH47 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 762.3 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 61 nm3

SASDH57 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 798.6 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 60 nm3

SASDH67 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 834.9 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 61 nm3

SASDH77 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 871.2 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 60 nm3

SASDH87 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 907.5 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 60 nm3

SASDH97 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 943.8 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 60 nm3

SASDHA7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 980.1 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 61 nm3

SASDHB7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1016.4 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 61 nm3

SASDHC7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1052.7 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 60 nm3

SASDHD7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1089 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 60 nm3

SASDHE7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1125.3 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 61 nm3

SASDHF7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1161.6 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.8 nm VolumePorod 61 nm3

SASDHG7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1197.9 Gy X-ray dose (J/kg) Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4 RgGuinier 2.7 nm VolumePorod 60 nm3