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6 hits found for Adamopoulos

SASDGP2 – Thymine dioxygenase full length J-DNA binding protein (JBP1)

Thymine dioxygenase JBP1 experimental SAS data
Thymine dioxygenase JBP1 Kratky plot
Sample: Thymine dioxygenase JBP1 monomer, 93 kDa Leishmania tarentolae protein
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 128 nm3

SASDGQ2 – Thymine dioxygenase lacking the JDNA binding domain Delta-JDBD (Δ-JDBD)

Delta-JDBD experimental SAS data
Delta-JDBD Kratky plot
Sample: Delta-JDBD monomer, 72 kDa Leismania tarentolae protein
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 3.1 nm
Dmax 9.9 nm
VolumePorod 108 nm3

SASDGR2 – Thymine dioxygenase J-containing DNA binding domain (JDBD)

J-DNA binding domain experimental SAS data
J-DNA binding domain Kratky plot
Sample: J-DNA binding domain monomer, 21 kDa Leishmania tarentolae protein
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 4
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 2.2 nm
Dmax 7.1 nm
VolumePorod 38 nm3

SASDGS2 – J-23-DNA

J-DNA (23mer) experimental SAS data
J-DNA (23mer) Kratky plot
Sample: J-DNA (23mer) monomer, 14 kDa DNA
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 2.3 nm
Dmax 7.3 nm
VolumePorod 20 nm3

SASDGT2 – Thymine dioxygenase J-containing DNA binding domain in complex with J-23-DNA (JDBD:J-23-DNA)

J-DNA binding domainJ-DNA (23mer) experimental SAS data
J-DNA binding domain J-DNA (23mer) Kratky plot
Sample: J-DNA binding domain monomer, 21 kDa Leishmania tarentolae protein
J-DNA (23mer) monomer, 14 kDa DNA
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 2.5 nm
Dmax 8.6 nm
VolumePorod 43 nm3

SASDGU2 – Thymine dioxygenase full length J-DNA binding protein in complex with J-23-DNA (JBP1:J-23-DNA)

Thymine dioxygenase JBP1J-DNA (23mer) experimental SAS data
Thymine dioxygenase JBP1 J-DNA (23mer) Kratky plot
Sample: Thymine dioxygenase JBP1 monomer, 93 kDa Leishmania tarentolae protein
J-DNA (23mer) monomer, 14 kDa DNA
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 4.1 nm
Dmax 14.1 nm
VolumePorod 148 nm3