Search

  
Advanced search  

19 hits found for Bradshaw

SASDU85 – Wild-type phosphoserine phosphatase RsbU dimer

Phosphoserine phosphatase RsbU experimental SAS data
MULTIFOXS model
Sample: Phosphoserine phosphatase RsbU dimer, 77 kDa Bacillus subtilis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 24
A general mechanism for initiating the bacterial general stress response. Elife 13 (2025)
...Bradshaw N
RgGuinier 3.9 nm
Dmax 14.3 nm
VolumePorod 117 nm3

SASDU95 – Hyperactive variant of phosphoserine phosphatase RsbU (Q94L) bound to the activator serine/threonine-protein kinase RsbT (heterotetrameric complex)

Serine/threonine-protein kinase RsbTPhosphoserine phosphatase RsbU (Q94L) experimental SAS data
Serine/threonine-protein kinase RsbT Phosphoserine phosphatase RsbU (Q94L) Kratky plot
Sample: Serine/threonine-protein kinase RsbT dimer, 29 kDa Bacillus subtilis (strain … protein
Phosphoserine phosphatase RsbU (Q94L) dimer, 77 kDa Bacillus subtilis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 24
A general mechanism for initiating the bacterial general stress response. Elife 13 (2025)
...Bradshaw N
RgGuinier 3.5 nm
Dmax 12.3 nm
VolumePorod 119 nm3

SASDDP6 – Anti-CD32b Antibody Clone 6G08 Antibody Binding Fragment (6G08 F(ab))

Anti-CD32b Antibody Clone 6G08 Antibody Binding Fragment experimental SAS data
Anti-CD32b Antibody Clone 6G08 Antibody Binding Fragment Kratky plot
Sample: Anti-CD32b Antibody Clone 6G08 Antibody Binding Fragment monomer, 46 kDa Homo sapiens protein
Buffer: 50mM HEPES, 150mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 5
Evaluating Anti-CD32b F(ab) Conformation Using Molecular Dynamics and Small-Angle X-Ray Scattering. Biophys J 115(2):289-299 (2018)
...Bradshaw RT, Orr CM, Frendéus B, Larsson G, Teige I, Cragg MS, Tews I, Essex JW
RgGuinier 2.6 nm
Dmax 7.3 nm
VolumePorod 68 nm3

SASDU77 – Human IgG1 F(ab') 5C05

Human IgG1 F(ab') 5C05 experimental SAS data
Human IgG1 F(ab') 5C05 Kratky plot
Sample: Human IgG1 F(ab') 5C05 monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 May 9
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 2.5 nm
Dmax 7.7 nm
VolumePorod 59 nm3

SASDU87 – Deglycosylated Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 5C05 F(ab')

Low affinity immunoglobulin gamma Fc region receptor II-bHuman IgG1 F(ab') 5C05 experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein
Human IgG1 F(ab') 5C05 monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 May 18
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 95 nm3

SASDU97 – Human IgG1 F(ab') 6C11

Human IgG1 F(ab') 6C11 experimental SAS data
Human IgG1 F(ab') 6C11 Kratky plot
Sample: Human IgG1 F(ab') 6C11 monomer, 24 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 May 9
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 2.5 nm
Dmax 7.6 nm
VolumePorod 56 nm3

SASDUA7 – Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 6C11 F(ab')

Low affinity immunoglobulin gamma Fc region receptor II-bHuman IgG1 F(ab') 6C11 experimental SAS data
Low affinity immunoglobulin gamma Fc region receptor II-b Human IgG1 F(ab') 6C11 Kratky plot
Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein
Human IgG1 F(ab') 6C11 monomer, 24 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 May 9
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 110 nm3

SASDUB7 – Human IgG1 F(ab') 6G11

Human IgG1 F(ab') 6G11 experimental SAS data
Human IgG1 F(ab') 6G11 Kratky plot
Sample: Human IgG1 F(ab') 6G11 monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 5
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 60 nm3

SASDUC7 – Deglycosylated Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 6G11 F(ab')

Low affinity immunoglobulin gamma Fc region receptor II-bHuman IgG1 F(ab') 6G11 experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein
Human IgG1 F(ab') 6G11 monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 9
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 3.5 nm
Dmax 13.5 nm
VolumePorod 97 nm3

SASDUD7 – Human IgG1 F(ab') 7C07

Human IgG1 F(ab') 7C07 experimental SAS data
Human IgG1 F(ab') 7C07 Kratky plot
Sample: Human IgG1 F(ab') 7C07 monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 5
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 2.6 nm
Dmax 8.2 nm
VolumePorod 59 nm3

SASDUE7 – Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 7C07 F(ab')

Low affinity immunoglobulin gamma Fc region receptor II-bHuman IgG1 F(ab') 7C07 experimental SAS data
Low affinity immunoglobulin gamma Fc region receptor II-b Human IgG1 F(ab') 7C07 Kratky plot
Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein
Human IgG1 F(ab') 7C07 monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 5
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 3.6 nm
Dmax 13.5 nm
VolumePorod 110 nm3

SASDUF7 – Deglycosylated Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 6G08 F(ab')

Low affinity immunoglobulin gamma Fc region receptor II-bHuman IgG1 F(ab') 6G08 experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein
Human IgG1 F(ab') 6G08 monomer, 24 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 14
Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling. Immunity (2026)
...Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pe...
RgGuinier 3.3 nm
Dmax 12.0 nm
VolumePorod 96 nm3

SASDDK7 – Disrupted-in-Schizophrenia 1 protein (DISC1 12d2)

Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 experimental SAS data
GASBOR model
Sample: Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 monomer, 19 kDa protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 1
Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein. PLoS One 13(1):e0191162 (2018)
...Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C
RgGuinier 2.6 nm
Dmax 7.3 nm
VolumePorod 41 nm3

SASDDL7 – anti-DISC1 single-domain camelid antibody VHH B5

anti-DISC1 single-domain camelid antibody VHH B5 experimental SAS data
GASBOR model
Sample: anti-DISC1 single-domain camelid antibody VHH B5 monomer, 14 kDa protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 19
Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein. PLoS One 13(1):e0191162 (2018)
...Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C
RgGuinier 1.8 nm
Dmax 8.4 nm
VolumePorod 21 nm3

SASDDM7 – Complex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and anti-DISC1 single-domain camelid antibody VHH B5

anti-DISC1 single-domain camelid antibody VHH B5Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 experimental SAS data
DAMMIF model
Sample: anti-DISC1 single-domain camelid antibody VHH B5 monomer, 14 kDa protein
Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 monomer, 19 kDa protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 19
Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein. PLoS One 13(1):e0191162 (2018)
...Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C
RgGuinier 3.1 nm
Dmax 10.4 nm
VolumePorod 77 nm3

SASDUT7 – The tandem SH2 domains of Tyrosine-protein kinase SYK

Tyrosine-protein kinase SYK experimental SAS data
Tyrosine-protein kinase SYK Kratky plot
Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13
The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures Structure (2024)
Bradshaw W, Harris G, Gileadi O, Katis V
RgGuinier 2.2 nm
Dmax 6.9 nm
VolumePorod 49 nm3

SASDUU7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound FCER1G diphospho-ITAM peptide

Tyrosine-protein kinase SYKHigh affinity immunoglobulin epsilon receptor subunit gamma experimental SAS data
Tyrosine-protein kinase SYK High affinity immunoglobulin epsilon receptor subunit gamma Kratky plot
Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein
High affinity immunoglobulin epsilon receptor subunit gamma monomer, 2 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13
The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures Structure (2024)
Bradshaw W, Harris G, Gileadi O, Katis V
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 54 nm3

SASDUV7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound CD3G diphospho-ITAM peptide

Tyrosine-protein kinase SYKT-cell surface glycoprotein CD3 gamma chain experimental SAS data
Tyrosine-protein kinase SYK T-cell surface glycoprotein CD3 gamma chain Kratky plot
Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein
T-cell surface glycoprotein CD3 gamma chain monomer, 3 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13
The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures Structure (2024)
Bradshaw W, Harris G, Gileadi O, Katis V
RgGuinier 2.1 nm
Dmax 7.1 nm
VolumePorod 56 nm3

SASDUW7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound TYROBP diphospho-ITAM peptide

Tyrosine-protein kinase SYKTYRO protein tyrosine kinase-binding protein experimental SAS data
Tyrosine-protein kinase SYK TYRO protein tyrosine kinase-binding protein Kratky plot
Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein
TYRO protein tyrosine kinase-binding protein monomer, 2 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13
The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures Structure (2024)
Bradshaw W, Harris G, Gileadi O, Katis V
RgGuinier 2.1 nm
Dmax 7.1 nm
VolumePorod 53 nm3