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7 hits found for Crosas

SASDJF3 – Aldo-keto reductase family 1 member B1 (AKR1B1) in the presence of 100 mM 5-methyl uridine

Aldo-keto reductase family 1 member B1 experimental SAS data
Aldo-keto reductase family 1 member B1 Kratky plot
Sample: Aldo-keto reductase family 1 member B1 monomer, 36 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, 100 mM 5-methyl uridine, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 14
Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples. Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020)
...Crosas E, Malfois M, Juanhuix J
RgGuinier 2.0 nm
Dmax 6.5 nm
VolumePorod 52 nm3

SASDJG3 – Bovine Liver Catalase in presence of 100 mM 5-methyl uridine

Catalase experimental SAS data
Catalase Kratky plot
Sample: Catalase tetramer, 240 kDa Bos taurus protein
Buffer: 50 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 14
Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples. Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020)
...Crosas E, Malfois M, Juanhuix J
RgGuinier 3.7 nm
Dmax 11.5 nm
VolumePorod 265 nm3

SASDJH3 – Hen Egg White Lysozyme in presence of 100 mM 5-methyl uridine

Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 16 kDa Gallus gallus protein
Buffer: 40 mM sodium acetate, 150 mM NaCl, 100 mM 5-methyl uridine, pH: 3.8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 7
Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples. Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020)
...Crosas E, Malfois M, Juanhuix J
RgGuinier 1.5 nm
Dmax 4.2 nm
VolumePorod 21 nm3

SASDJJ3 – SH3 domain of alpha spectrin (amino acids 965-1025) in presence of 100 mM 5-methyl uridine

Spectrin alpha chain, non-erythrocytic 1 experimental SAS data
Spectrin alpha chain, non-erythrocytic 1 Kratky plot
Sample: Spectrin alpha chain, non-erythrocytic 1 , 7 kDa Gallus gallus protein
Buffer: 10 mM citric acid, 150 mM NaCl, 100 mM 5-methyl uridine, pH: 3
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 14
Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples. Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020)
...Crosas E, Malfois M, Juanhuix J
RgGuinier 1.3 nm
Dmax 4.5 nm
VolumePorod 10 nm3

SASDDQ5 – Human metaphase chromosomes in 17mM magnesium chloride

Metaphase chromosomes in 17 mM magnesium chloride experimental SAS data
Metaphase chromosomes in 17 mM magnesium chloride Kratky plot
Sample: Metaphase chromosomes in 17 mM magnesium chloride monomer, 0 kDa Homo sapiens
Buffer: 10 mM PIPES, 17 mM magnesium chloride, 20 mM sodium chloride, 120 mM potassium chloride, 40% glycerol, pH: 7.2
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 May 23
Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure. EMBO J 38(7) (2019)
...Crosas E, Otón J, Melero R, Engel BD, Daban JR

SASDDR5 – Human metaphase chromosomes in 5 mM magnesium chloride

Human metaphase chromosomes in 5 mM magnesium chloride experimental SAS data
Human metaphase chromosomes in 5 mM magnesium chloride Kratky plot
Sample: Human metaphase chromosomes in 5 mM magnesium chloride monomer, 0 kDa Homo sapiens
Buffer: 10 mM PIPES, 5 mM magnesium chloride, 10 mM sodium chloride, 120 mM potassium chloride, 40% glycerol, pH: 7.2
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 Jun 6
Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure. EMBO J 38(7) (2019)
...Crosas E, Otón J, Melero R, Engel BD, Daban JR

SASDDS5 – Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride

Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride experimental SAS data
Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride Kratky plot
Sample: Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride monomer, 0 kDa Homo sapiens
Buffer: 10 mM PIPES, hexaamminecobalt(III) chloride, 40% glycerol, pH: 7.2
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 Sep 12
Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure. EMBO J 38(7) (2019)
...Crosas E, Otón J, Melero R, Engel BD, Daban JR