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34 hits found for Fernandes

SASDTG2 – LCP domain of LytR from Streptococcus dysgalactiae in the presence of geranylgeranyl diphosphate (150 mM NaCl)

Biofilm regulatory protein experimental SAS data
Biofilm regulatory protein Kratky plot
Sample: Biofilm regulatory protein monomer, 34 kDa Streptococcus dysgalactiae subsp. … protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 1
Structural insights of an LCP protein-LytR-from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods. Front Chem 12:1379914 (2024)
...Fernandes HS, Muthukumaran J, Ramos J, Bryton J, Panjkovich A, Svergun D, Santos MFA, Correia MAS, Fernandes AR, Romão MJ, Sousa SF, Santos-Silva T
RgGuinier 2.5 nm
Dmax 8.0 nm
VolumePorod 83 nm3

SASDTH2 – LCP domain of LytR from Streptococcus dysgalactiae in the absence of ligand (150 mM NaCl)

Biofilm regulatory protein experimental SAS data
Biofilm regulatory protein Kratky plot
Sample: Biofilm regulatory protein monomer, 34 kDa Streptococcus dysgalactiae subsp. … protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 1
Structural insights of an LCP protein-LytR-from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods. Front Chem 12:1379914 (2024)
...Fernandes HS, Muthukumaran J, Ramos J, Bryton J, Panjkovich A, Svergun D, Santos MFA, Correia MAS, Fernandes AR, Romão MJ, Sousa SF, Santos-Silva T
RgGuinier 2.4 nm
Dmax 9.0 nm
VolumePorod 76 nm3

SASDRN5 – Nucleolar RNA Chaperone-Like Protein 1 (NURC1)

AT5g04600/T32M21_200 experimental SAS data
GASBOR model
Sample: AT5g04600/T32M21_200 monomer, 25 kDa Arabidopsis thaliana protein
Buffer: 50 mM HNa2PO4, 300 mM NaCl, 5% glycerol (v/v), 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 22
Structural and functional analysis of a plant nucleolar RNA chaperone-like protein. Sci Rep 13(1):9656 (2023)
Fernandes R, Ostendorp A, Ostendorp S, Mehrmann J, Falke S, Graewert MA, Weingartner M, Kehr J, Hoth S
RgGuinier 3.5 nm
Dmax 12.4 nm
VolumePorod 66 nm3

SASDTE5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 26 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 82 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.7 nm
Dmax 23.0 nm
VolumePorod 160 nm3

SASDXH8 – Leukocyte Immunoglobulin-Like Receptor LILR-B3

Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) experimental SAS data
MODELLER model
Sample: Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) monomer, 47 kDa Homo sapiens protein
Buffer: 20mM HEPES, 140mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Oct 5
Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity. Nat Commun (2026)
...Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ
RgGuinier 3.6 nm
Dmax 13.5 nm
VolumePorod 137 nm3

SASDVA4 – β-Antigen C Protein B6C domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

IgA FC receptorLeukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) experimental SAS data
MULTIFOXS model
Sample: IgA FC receptor monomer, 20 kDa Streptococcus agalactiae protein
Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) monomer, 47 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Nov 17
Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity. Nat Commun (2026)
...Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ
RgGuinier 3.9 nm
Dmax 13.5 nm
VolumePorod 202 nm3

SASDTF5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 49 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.8 nm
Dmax 24.0 nm
VolumePorod 181 nm3

SASDVB4 – β-Antigen C Protein B75KN-CTD domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R)IgA FC receptor (N770H) experimental SAS data
MULTIFOXS model
Sample: Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) monomer, 47 kDa Homo sapiens protein
IgA FC receptor (N770H) monomer, 12 kDa Streptococcus agalactiae protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Nov 17
Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity. Nat Commun (2026)
...Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ
RgGuinier 3.9 nm
Dmax 14.1 nm
VolumePorod 175 nm3

SASDTG5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 103 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 6.2 nm
Dmax 25.0 nm
VolumePorod 215 nm3

SASDTH5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 206 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 6.8 nm
Dmax 28.0 nm
VolumePorod 578 nm3

SASDXG8 – Streptococcus agalactiae IgA FC receptor

IgA FC receptor β-Antigen C Protein experimental SAS data
FOXSDOCK model
Sample: IgA FC receptor β-Antigen C Protein monomer, 71 kDa Streptococcus agalactiae protein
Buffer: 20mM HEPES, 140mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Oct 5
Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity. Nat Commun (2026)
...Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ
RgGuinier 5.9 nm
Dmax 20.2 nm
VolumePorod 140 nm3

SASDTJ5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 299 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein hexamer, 245 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 7.1 nm
Dmax 29.0 nm
VolumePorod 708 nm3

SASDXF8 – β-Antigen C Protein bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

IgA FC receptor β-Antigen C ProteinLeukocyte immunoglobulin-like receptor subfamily B3 experimental SAS data
FOXSDOCK model
Sample: IgA FC receptor β-Antigen C Protein monomer, 71 kDa Streptococcus agalactiae protein
Leukocyte immunoglobulin-like receptor subfamily B3 monomer, 94 kDa Homo sapiens protein
Buffer: 20mM HEPES, 140mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Oct 5
Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity. Nat Commun (2026)
...Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ
RgGuinier 7.0 nm
Dmax 26.5 nm
VolumePorod 447 nm3

SASDTX5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 19 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 6.3 nm
Dmax 26.0 nm
VolumePorod 233 nm3

SASDTY5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 50 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 6.9 nm
Dmax 28.5 nm
VolumePorod 571 nm3

SASDTZ5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 103 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein hexamer, 281 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 7.6 nm
Dmax 32.0 nm
VolumePorod 764 nm3

SASDT26 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 216 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein octamer, 375 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 8.2 nm
Dmax 33.0 nm
VolumePorod 1028 nm3

SASDSZ9 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 238 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 3.2 nm
Dmax 14.0 nm
VolumePorod 19 nm3

SASDT22 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 476 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 3.7 nm
Dmax 16.0 nm
VolumePorod 33 nm3

SASDT32 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 952 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 17 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 4.1 nm
Dmax 18.0 nm
VolumePorod 53 nm3

SASDVS6 – SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245): 1666.7 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 17 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 4.5 nm
Dmax 19.0 nm
VolumePorod 117 nm3

SASDT42 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 238 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 2.4 nm
Dmax 11.0 nm
VolumePorod 10 nm3

SASDT52 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 476 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 12 nm3

SASDT62 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 952 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 2.7 nm
Dmax 12.0 nm
VolumePorod 13 nm3

SASDVT6 – SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 14 nm3

SASDST9 – SARS-CoV-2 N-protein (N1-245; residues 1-245): 294.7 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 110 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.2 nm
Dmax 22.0 nm
VolumePorod 117 nm3

SASDSR9 – SARS-CoV-2 N-protein (N1-245; residues 1-245): 38.2 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 3.3 nm
Dmax 15.5 nm
VolumePorod 56 nm3

SASDSS9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 76.4 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 55 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 4.0 nm
Dmax 18.0 nm
VolumePorod 67 nm3

SASDSU9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 400 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 110 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.4 nm
Dmax 24.0 nm
VolumePorod 157 nm3

SASDSV9 – SARS-CoV-2 N-protein (N1-245; residues 1-245) L223P, L227P and L230P triple mutant: 36.4 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 3.2 nm
Dmax 13.5 nm
VolumePorod 53 nm3

SASDSW9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 72.7 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 3.5 nm
Dmax 14.5 nm
VolumePorod 47 nm3

SASDVU6 – SARS-CoV-2 N-protein (N1-245; amino acids 1-245): 167 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 55 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.2 nm
Dmax 20.5 nm
VolumePorod 121 nm3

SASDSX9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 294 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 4.4 nm
Dmax 19.0 nm
VolumePorod 68 nm3

SASDSY9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 400 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
...Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 4.6 nm
Dmax 19.0 nm
VolumePorod 75 nm3