Synchrotron SAXS
data from solutions of
Full-length tag-free Four and a half LIM domains protein 2 (FHL2), 7.5 mg/ml
in
20 mM HEPES, 150 mM NaCl, 5 % (v/v) glycerol, 10 mM β-mercaptoethanol, pH 7.4
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.137759 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 7.5 mg/ml was injected at a 0.70 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
3000 successive
0.995 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
