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9 hits found for Sarah Atkinson

SASDR65 – ATP-dependent RNA helicase DDX3X (amino acids 1-580)

ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580) experimental SAS data
ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580) Kratky plot
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580) monomer, 67 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 116 nm3

SASDR75 – ATP-dependent RNA helicase DDX3X (amino acids 50-580)

ATP-dependent RNA helicase DDX3X (truncation; amino acids 50-580) experimental SAS data
ATP-dependent RNA helicase DDX3X (truncation; amino acids 50-580) Kratky plot
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 50-580) monomer, 62 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.6 nm
Dmax 12.9 nm
VolumePorod 102 nm3

SASDR85 – ATP-dependent RNA helicase DDX3X (amino acids 100-580)

ATP-dependent RNA helicase DDX3X (truncation; amino acids 100-580) experimental SAS data
ATP-dependent RNA helicase DDX3X (truncation; amino acids 100-580) Kratky plot
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 100-580) monomer, 57 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.4 nm
Dmax 10.4 nm
VolumePorod 88 nm3

SASDR95 – ATP-dependent RNA helicase DDX3X (amino acids 135-580)

ATP-dependent RNA helicase DDX3X (truncation; amino acids 135-580) experimental SAS data
DAMFILT model
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 135-580) monomer, 52 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.1 nm
Dmax 9.2 nm
VolumePorod 77 nm3

SASDRA5 – 15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target)

15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) experimental SAS data
DAMMIF model
Sample: 15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) dimer, 10 kDa RNA
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 1.5 nm
Dmax 4.2 nm
VolumePorod 14 nm3

SASDRB5 – ATP-dependent RNA helicase DDX3X (amino acids 1-580) bound to a 15 nucleotide RNA duplex

ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580)15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) experimental SAS data
MONSA model
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580) monomer, 67 kDa Homo sapiens protein
15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) dimer, 10 kDa RNA
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.6 nm
Dmax 11.2 nm
VolumePorod 105 nm3

SASDRC5 – ATP-dependent RNA helicase DDX3X (amino acids 135-580) bound to a 15 nucleotide RNA duplex

ATP-dependent RNA helicase DDX3X (truncation; amino acids 135-580)15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) experimental SAS data
MONSA model
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 135-580) monomer, 52 kDa Homo sapiens protein
15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) dimer, 10 kDa RNA
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 2.9 nm
Dmax 8.5 nm

SASDDV5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum

4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 159 nm3

SASDDW5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum + pyruvate

4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, 5mM sodium pyruvate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.3 nm
Dmax 8.9 nm
VolumePorod 165 nm3