The extracellular domain of SaNSrFP binds bacitracin and allows the identification of new members of the BceAB transporter family

Jens Reiners.

SASDWZ6 – ABC transporter permease protein extracellular domain from S.agalactiae (SaNsrP ECD)

ABC transporter, permease protein, extracellular domain
MWI(0) 23 kDa
MWexpected 23 kDa
VPorod 50 nm3
log I(s) 2.29×101 2.29×100 2.29×10-1 2.29×10-2
ABC transporter, permease protein, extracellular domain small angle scattering data  s, nm-1
ln I(s)
ABC transporter, permease protein, extracellular domain Guinier plot ln 2.29×101 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
ABC transporter, permease protein, extracellular domain Kratky plot 1.104 0 √3 sRg
p(r)
ABC transporter, permease protein, extracellular domain pair distance distribution function Rg: 2.5 nm 0 Dmax: 9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of ABC transporter permease protein extracellular domain from S.agalactiae (SaNsrP ECD) in 25 mM MES, 500 mM NaCl, pH 6 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.65 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ABC transporter, permease protein, extracellular domain (SaNsrP ECD)
Mol. type   Protein
Organism   Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Olig. state   Monomer
Mon. MW   23.4 kDa
 
UniProt   Q8DZX0 (311-412)
Sequence   FASTA