A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism.

Büttner H, Perbandt M, Kohler T, Kikhney A Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H, mBio 11(5) (2020) Europe PMC

SASDJ92 – S. epidermidis extracellular matrix binding protein (Embp) F-repeats

Extracellular matrix binding protein F-repeats
MWI(0) 82 kDa
MWexpected 70 kDa
log I(s) 9.06×101 9.06×100 9.06×10-1 9.06×10-2
Extracellular matrix binding protein F-repeats small angle scattering data  s, nm-1
ln I(s)
Extracellular matrix binding protein F-repeats Guinier plot ln 9.07×101 Rg: 7.0 nm 0 (7.0 nm)-2 s2
(sRg)2I(s)/I(0)
Extracellular matrix binding protein F-repeats Kratky plot 1.104 0 √3 sRg
p(r)
Extracellular matrix binding protein F-repeats pair distance distribution function Rg: 7.7 nm 0 Dmax: 28 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Extracellular matrix binding protein F-repeats SASREF model
log I(s)
 s, nm-1
Extracellular matrix binding protein F-repeats GASBOR model
Synchrotron SAXS data from solutions of S. epidermidis extracellular matrix binding protein (Embp) F-repeats in 50 mM MES, 150 mM NaCl, pH 6 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.10 mg/ml was measured at 10.1°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Extracellular matrix binding protein F-repeats (rEmbp2588)
Mol. type   Protein
Organism   Staphylococcus epidermidis
Olig. state   Other
Mon. MW   70.4 kDa
 
UniProt   Q5HPA2
Sequence   FASTA
 
PDB ID   6GV8