SASDV84 – SH-01 in complex with Fab 3E12

SH-01
Fab 3E12

MW^experimental	91	kDa
MW^expected	91	kDa
V^Porod	124	nm³

log I(s) 4.98×10³ 4.98×10² 4.98×10¹ 4.98×10⁰

SH-01 Fab 3E12 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 4.98×10³ R_g: 3.8 nm 0 (3.8 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 4.0 nm 0 D_max: 14 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.8

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.487
0.969

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of SH-01 in complex with Fab 3E12 in 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 7.2 mg/ml was injected at a 0.30 ml/min flow rate onto a Cytiva Superdex 200 Increase 5/150 column at 20°C. 1800 successive 0.500 second frames were collected throughout the entire SEC column elution; 106 frames were measured across the sample elution peak and used for averaging. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SH-01
Mol. type		Protein
Olig. state		Monomer
Mon. MW		40.8 kDa
Sequence		FASTA

Fab 3E12
Mol. type		Protein
Organism		Mus musculus
Olig. state		Monomer
Mon. MW		50 kDa
Sequence		FASTA