SASDUP4 – Dimer of the N-terminal 50 residues of the H-NS family protein MvaT

H-NS family protein MvaT

MW^experimental	11	kDa
MW^expected	12	kDa

log I(s) 2.22×10^-2 2.22×10^-3 2.22×10^-4 2.22×10^-5

H-NS family protein MvaT small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 2.22×10^-2 R_g: 2.9 nm 0 (2.9 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

D_max: 14 nm

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

H-NS family protein MvaT PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of TurB_nt50 in 20 mM Tris-HCl 500 mM NaCl, 10% glycerol, 500 mM Imidazole, pH8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK; Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 1.01 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 80.00 μl sample at 14 mg/ml was injected at a 0.01 ml/min flow rate onto a GE Superdex 75 Increase 3.2/300 column at 20°C. 303 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The averaged scattering profile near the elution peak (frames 134–144) was calculated by MOLASS.

H-NS family protein MvaT (TurB_nt50)
Mol. type		Protein
Organism		Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Olig. state		Dimer
Mon. MW		5.8 kDa

UniProt		Q88GF9 (1-50)
Sequence		FASTA

PDB ID		8H8H