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34 hits found for Heat shock cognate 71 kDa protein

SASDL42 – ...Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70)

Heat shock cognate 71 kDa protein experimental SAS data
Human Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70) Rg histogram
Sample: Heat shock cognate 71 kDa protein monomer, 71 kDa Homo sapiens protein
Buffer: 25 mM Tris HCl, 50 mM NaCl, 5 mM Sodium Phosphate, 5 mM KCl, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 18
...71 kDa heat shock cognate protein (HSPA8/hHsc70) ...Proteins and Proteomics :140719 (2021)
Silva N, de Camargo Rodrigues L, Dores-Silva P, Montanari C, Ramos C, Barbosa L, Borges J
RgGuinier 3.7 nm
Dmax 11.5 nm
VolumePorod 111 nm3

SASDL52 – ...Heat Shock 70 kDa Protein 1A (Hsp70-1A/HSPA1A)

Heat shock 70 kDa protein 1A experimental SAS data
DAMMIN model
Sample: Heat shock 70 kDa protein 1A monomer, 70 kDa Homo sapiens protein
Buffer: 25 mM Tris HCl, 50 mM NaCl, 5 mM Sodium Phosphate, 5 mM KCl, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 18
...71 kDa heat shock cognate protein (HSPA8/hHsc70) ...Proteins and Proteomics :140719 (2021)
Silva N, de Camargo Rodrigues L, Dores-Silva P, Montanari C, Ramos C, Barbosa L, Borges J
RgGuinier 3.7 nm
Dmax 11.0 nm
VolumePorod 138 nm3

SASDYK4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (2 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.1 nm
Dmax 17.5 nm
VolumePorod 143 nm3

SASDYL4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (3 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.1 nm
Dmax 18.3 nm
VolumePorod 143 nm3

SASDYM4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (4 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.3 nm
Dmax 18.4 nm
VolumePorod 160 nm3

SASDYN4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (5 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.4 nm
Dmax 19.1 nm
VolumePorod 167 nm3

SASDYV4 – Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ADP (1 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.2 nm
Dmax 22.0 nm
VolumePorod 252 nm3

SASDYW4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (2 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.5 nm
Dmax 22.9 nm
VolumePorod 291 nm3

SASDYX4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (3 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.7 nm
Dmax 23.8 nm
VolumePorod 340 nm3

SASDYY4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (4 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.7 nm
Dmax 23.5 nm
VolumePorod 317 nm3

SASDYZ4 – Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (7 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.9 nm
Dmax 25.5 nm
VolumePorod 334 nm3

SASDY25 – Heat shock cognate 71 kDa protein (Hsc70) T204A APO (0.5 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.6 nm
Dmax 24.0 nm
VolumePorod 324 nm3

SASDY35 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (2 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.8 nm
Dmax 25.0 nm
VolumePorod 351 nm3

SASDY45 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (3 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.6 nm
Dmax 25.0 nm
VolumePorod 348 nm3

SASDY55 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (4 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.7 nm
Dmax 24.5 nm
VolumePorod 351 nm3

SASDY65 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (5 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.7 nm
Dmax 24.7 nm
VolumePorod 352 nm3

SASDY75 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (7 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data
Heat shock cognate 71 kDa protein Kratky plot
Sample: Heat shock cognate 71 kDa protein , 71 kDa protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.6 nm
Dmax 25.0 nm
VolumePorod 360 nm3

SASDY85 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ATP (1 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 3.6 nm
Dmax 15.3 nm
VolumePorod 122 nm3

SASDY95 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ATP (2 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 3.7 nm
Dmax 16.4 nm
VolumePorod 131 nm3

SASDYA5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ATP (3 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 3.9 nm
Dmax 16.7 nm
VolumePorod 141 nm3

SASDYB5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ATP (4 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.3 nm
Dmax 16.7 nm
VolumePorod 149 nm3

SASDYD5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ATP (7 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.2 nm
Dmax 18.1 nm
VolumePorod 162 nm3

SASDYE5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (1 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.4 nm
Dmax 20.3 nm
VolumePorod 169 nm3

SASDYF5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (2 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.8 nm
Dmax 22.3 nm
VolumePorod 203 nm3

SASDYG5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (3 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.9 nm
Dmax 22.0 nm
VolumePorod 218 nm3

SASDYH5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (4 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.0 nm
Dmax 22.8 nm
VolumePorod 226 nm3

SASDYJ5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (7 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.5 nm
Dmax 24.4 nm
VolumePorod 250 nm3

SASDYK5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A apo (1 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 4.8 nm
Dmax 21.0 nm
VolumePorod 195 nm3

SASDYL5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A apo (2 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.0 nm
Dmax 22.0 nm
VolumePorod 222 nm3

SASDYM5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A apo (3 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.3 nm
Dmax 23.0 nm
VolumePorod 235 nm3

SASDYN5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A apo (4 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.2 nm
Dmax 24.0 nm
VolumePorod 245 nm3

SASDYP5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A apo (5 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.4 nm
Dmax 23.5 nm
VolumePorod 254 nm3

SASDYQ5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A apo (7 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.5 nm
Dmax 25.0 nm
VolumePorod 267 nm3

SASDYR5 – ...Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (5 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein experimental SAS data
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot
Sample: ...Heat shock cognate 71 kDa protein , 71 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A
RgGuinier 5.3 nm
Dmax 22.8 nm
VolumePorod 236 nm3