SASDXF8 – β-Antigen C Protein bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

Synchrotron small-angle X-ray scattering (SAXS) data for β-Antigen C protein in complex with two molecules of leukocyte immunoglobulin-like receptor LILR-B3 were collected at SIBYLS beamline 12.3.1 of the Advanced Light Source in SEC-SAXS mode. In-line size-exclusion chromatography (SEC) was performed using an Agilent 1260 HPLC system. The SEC conditions were as follows: a 60 µL sample at 5 mg/mL was injected onto a Shodex KW-803 column at 4 °C that had been previously equilibrated in 20 mM HEPES, 140 mM NaCl, pH 7.4, and eluted at 0.65 mL/min. Scattering was recorded on a Pilatus3 X 2M detector at a sample-to-detector distance of 2.1 m and a wavelength of λ = 0.1127 nm (I(q) vs q; q = 4π sin θ/λ, where 2θ is the scattering angle). 913 successive 2-s frames were collected. The scattering curves were normalized to the transmitted-beam intensity, radially averaged, and corrected by subtracting the solvent blank.