| MWexperimental | 177 | kDa |
| MWexpected | 165 | kDa |
| VPorod | 447 | nm3 |
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log I(s)
6.65×101
6.65×100
6.65×10-1
6.65×10-2
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s, nm-1
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Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.
Rumpret M, Lewis Marffy AL, Zhang Y van Woudenbergh E, van der Lans SPA, Xu X, Fu Z, Zhao Y, Catton EA, Paré G, McGregor F, Spiller OB, Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ, Nat Commun (2026) Europe PMCSASDXF8 – β-Antigen C Protein bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3
IgA FC receptor β-Antigen C ProteinLeukocyte immunoglobulin-like receptor subfamily B3
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Fits and models
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Synchrotron small-angle X-ray scattering (SAXS) data for β-Antigen C protein in complex with two molecules of leukocyte immunoglobulin-like receptor LILR-B3 were collected at SIBYLS beamline 12.3.1 of the Advanced Light Source in SEC-SAXS mode. In-line size-exclusion chromatography (SEC) was performed using an Agilent 1260 HPLC system. The SEC conditions were as follows: a 60 µL sample at 5 mg/mL was injected onto a Shodex KW-803 column at 4 °C that had been previously equilibrated in 20 mM HEPES, 140 mM NaCl, pH 7.4, and eluted at 0.65 mL/min. Scattering was recorded on a Pilatus3 X 2M detector at a sample-to-detector distance of 2.1 m and a wavelength of λ = 0.1127 nm (I(q) vs q; q = 4π sin θ/λ, where 2θ is the scattering angle). 913 successive 2-s frames were collected. The scattering curves were normalized to the transmitted-beam intensity, radially averaged, and corrected by subtracting the solvent blank. |
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