| MWI(0) | 62 | kDa |
| MWexpected | 53 | kDa |
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log I(s)
4.50×10-2
4.50×10-3
4.50×10-4
4.50×10-5
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s, nm-1
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Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
Sharma M, Aswal V, Kumar V, Chidambaram R, IUCrJ 7(2) (2020) DOISASDFA7 – Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O
Lysinibacillus Mosquito-larvicidal receptor binding component|
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Fits and models
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SANS
data from solutions of
Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O
in
25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH 7.5
were collected
using a 1 m long He3 position sensitive detector detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.52 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 3.30 mg/ml was measured
at 25°C.
One
43200 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Experimental Molecular weight Value estimated using I(0) values from GNOM analysis. Intensity has been normalized to protein concentration of 1 mg/ml. Actual sample used was 3.3 mg/ml. |
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