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8
hits found for
Nucleoprotein (L223P, L227P, L230P)
SASDVT6
– SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM
Sample:
Nucleoprotein
(L223P,
L227P,
L230P)
monomer, 8 kDa
Severe acute respiratory …
protein
Buffer:
100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:
SAXS data collected at BM29, ESRF
on 2023 Jan 29
SARS-CoV-2 N-protein variants: IDL176-246
Guillem Hernandez
R
g
Guinier
2.8
nm
D
max
12.0
nm
Volume
Porod
14
nm
3
SASDT42
– SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 238 µM
Sample:
Nucleoprotein
(L223P,
L227P,
L230P)
monomer, 8 kDa
Severe acute respiratory …
protein
Buffer:
100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:
SAXS data collected at BM29, ESRF
on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
R
g
Guinier
2.4
nm
D
max
11.0
nm
Volume
Porod
10
nm
3
SASDT52
– SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 476 µM
Sample:
Nucleoprotein
(L223P,
L227P,
L230P)
monomer, 8 kDa
Severe acute respiratory …
protein
Buffer:
100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:
SAXS data collected at BM29, ESRF
on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
R
g
Guinier
2.7
nm
D
max
11.0
nm
Volume
Porod
12
nm
3
SASDT62
– SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 952 µM
Sample:
Nucleoprotein
(L223P,
L227P,
L230P)
monomer, 8 kDa
Severe acute respiratory …
protein
Buffer:
100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:
SAXS data collected at BM29, ESRF
on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
R
g
Guinier
2.7
nm
D
max
12.0
nm
Volume
Porod
13
nm
3
SASDSV9
– SARS-CoV-2 N-protein (N1-245; residues 1-245) L223P, L227P and L230P triple mutant: 36.4 µM
Sample:
Nucleoprotein
(L223P,
L227P,
L230P)
monomer, 27 kDa
Severe acute respiratory …
protein
Buffer:
100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:
SAXS data collected at B21, Diamond Light Source
on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
R
g
Guinier
3.2
nm
D
max
13.5
nm
Volume
Porod
53
nm
3
SASDSW9
– SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 72.7 µM
Sample:
Nucleoprotein
(L223P,
L227P,
L230P)
monomer, 27 kDa
Severe acute respiratory …
protein
Buffer:
100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:
SAXS data collected at B21, Diamond Light Source
on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
R
g
Guinier
3.5
nm
D
max
14.5
nm
Volume
Porod
47
nm
3
SASDSX9
– SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 294 µM
Sample:
Nucleoprotein
(L223P,
L227P,
L230P)
monomer, 27 kDa
Severe acute respiratory …
protein
Buffer:
100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:
SAXS data collected at B21, Diamond Light Source
on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
R
g
Guinier
4.4
nm
D
max
19.0
nm
Volume
Porod
68
nm
3
SASDSY9
– SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 400 µM
Sample:
Nucleoprotein
(L223P,
L227P,
L230P)
monomer, 27 kDa
Severe acute respiratory …
protein
Buffer:
100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:
SAXS data collected at B21, Diamond Light Source
on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
R
g
Guinier
4.6
nm
D
max
19.0
nm
Volume
Porod
75
nm
3
