Non Structural Protein Sars CoV-2

Hira Singh Gariya.

SASDXA6 – Non Structural Protein 9 (ORF1ab polyprotein) from SARS CoV-2

ORF1ab polyprotein
MWI(0) 623 kDa
MWexpected 22 kDa
VPorod 66 nm3
log I(s) 4.26×10-1 4.26×10-2 4.26×10-3 4.26×10-4
ORF1ab polyprotein small angle scattering data  s, nm-1
ln I(s)
ORF1ab polyprotein Guinier plot ln 4.26×10-1 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
ORF1ab polyprotein Kratky plot 1.104 0 √3 sRg
p(r)
ORF1ab polyprotein pair distance distribution function Rg: 2.6 nm 0 Dmax: 6.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
ORF1ab polyprotein PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
SAXS data from solutions of Non Structural Protein 9 (ORF1ab polyprotein) from SARS CoV-2 in 50mM Tris 300mM Nacl, pH 8 were collected on the Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, Lucknow, India) using a Mythen2 R 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 10°C. Three successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ORF1ab polyprotein (NSP9)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Dimer
Mon. MW   11.2 kDa
 
UniProt   A0A679G4D8 (4393-4492)
Sequence   FASTA
 
PDB ID   6W4B