Small Angle Scattering Biological Data Bank SASBDB

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14 hits found for Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)

SASDSY7 – ...insensitive 2, KAI2 protein (monomer peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.3	nm
D_max	9.5	nm
Volume^Porod	58	nm³

SASDSZ7 – ...insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (monomer peak)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.9	nm
D_max	13.9	nm
Volume^Porod	60	nm³

SASDS28 – ...insensitive 2, KAI2 protein (aggregate peak)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	4.1	nm
D_max	15.8	nm
Volume^Porod	283	nm³

SASDS38 – ...insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (aggregate peak)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	4.9	nm
D_max	20.0	nm
Volume^Porod	345	nm³

SASDS48 – ...insensitive 2, KAI2 protein mutant S95A (monomer peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot

Sample:	Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants

R_g^Guinier	1.9	nm
D_max	6.3	nm
Volume^Porod	55	nm³

SASDS58 – ...insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (monomer peak)

Sample:	Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.1	nm
D_max	7.2	nm
Volume^Porod	61	nm³

SASDS68 – ...insensitive 2, KAI2 protein mutant S95A (aggregate peak)

Sample:	Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	3.9	nm
D_max	13.2	nm
Volume^Porod	171	nm³

SASDS78 – ...insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (aggregate peak)

Sample:	Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	4.5	nm
D_max	19.3	nm
Volume^Porod	285	nm³

SASDS88 – ...insensitive 2, KAI2 protein mutant S95C (monomer peak)

Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) experimental SAS data

Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) Kratky plot

Sample:	Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	1.9	nm
D_max	5.7	nm
Volume^Porod	45	nm³

SASDS98 – ...insensitive 2, KAI2 protein mutant S95C with a synthetic strigolactone analogue ligand (monomer peak)

Sample:	Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	1.9	nm
D_max	5.7	nm
Volume^Porod	35	nm³

SASDSA8 – ...insensitive 2, KAI2 protein mutant H246Y (monomer peak)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot

Sample:	Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	3.0	nm
D_max	16.1	nm
Volume^Porod	70	nm³

SASDSB8 – ...insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (monomer peak)

Sample:	Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.9	nm
D_max	13.2	nm
Volume^Porod	68	nm³

SASDSC8 – ...insensitive 2, KAI2 protein mutant H246Y (aggregate peak)

Sample:	Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	5.6	nm
D_max	23.0	nm
Volume^Porod	494	nm³

SASDSD8 – ...insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (aggregate peak)

Sample:	Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

...KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	5.1	nm
D_max	19.3	nm
Volume^Porod	403	nm³