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14 hits found for Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)

SASDSY7 – ...insensitive 2, KAI2 protein (monomer peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 58 nm3

SASDSZ7 – ...insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.9 nm
Dmax 13.9 nm
VolumePorod 60 nm3

SASDS28 – ...insensitive 2, KAI2 protein (aggregate peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 283 nm3

SASDS38 – ...insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (aggregate peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.9 nm
Dmax 20.0 nm
VolumePorod 345 nm3

SASDS48 – ...insensitive 2, KAI2 protein mutant S95A (monomer peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
RgGuinier 1.9 nm
Dmax 6.3 nm
VolumePorod 55 nm3

SASDS58 – ...insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.1 nm
Dmax 7.2 nm
VolumePorod 61 nm3

SASDS68 – ...insensitive 2, KAI2 protein mutant S95A (aggregate peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 3.9 nm
Dmax 13.2 nm
VolumePorod 171 nm3

SASDS78 – ...insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (aggregate peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.5 nm
Dmax 19.3 nm
VolumePorod 285 nm3

SASDS88 – ...insensitive 2, KAI2 protein mutant S95C (monomer peak)

Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) experimental SAS data
Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) Kratky plot
Sample: Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 1.9 nm
Dmax 5.7 nm
VolumePorod 45 nm3

SASDS98 – ...insensitive 2, KAI2 protein mutant S95C with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) experimental SAS data
Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) Kratky plot
Sample: Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 1.9 nm
Dmax 5.7 nm
VolumePorod 35 nm3

SASDSA8 – ...insensitive 2, KAI2 protein mutant H246Y (monomer peak)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data
Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot
Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 3.0 nm
Dmax 16.1 nm
VolumePorod 70 nm3

SASDSB8 – ...insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data
Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot
Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.9 nm
Dmax 13.2 nm
VolumePorod 68 nm3

SASDSC8 – ...insensitive 2, KAI2 protein mutant H246Y (aggregate peak)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data
Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot
Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 5.6 nm
Dmax 23.0 nm
VolumePorod 494 nm3

SASDSD8 – ...insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (aggregate peak)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data
Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot
Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
...KAI2 catalytic mutants
Sabrina Davies
RgGuinier 5.1 nm
Dmax 19.3 nm
VolumePorod 403 nm3