Small Angle Scattering Biological Data Bank SASBDB


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13 hits found for Proliferating cell nuclear antigen

SASDED6 – Proliferating cell nuclear antigen - UbPCNA - Split Fusion Trimer

Proliferating cell nuclear antigen experimental SAS data

Sample:	Proliferating cell nuclear antigen trimer, 117 kDa Saccharomyces cerevisiae protein
Buffer:	20 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6

Conformational Flexibility of Ubiquitin-Modified and SUMO-Modified PCNA Shown by Full-Ensemble Hybrid Methods. J Mol Biol 430(24):5294-5303 (2018)
Powers KT, Lavering ED, Washington MT

R_g^Guinier	4.1	nm
D_max	14.0	nm
Volume^Porod	238	nm³

SASDEE6 – Proliferating cell nuclear antigen - SUMOPCNA - Split Fusion Trimer

Sample:	Proliferating cell nuclear antigen trimer, 125 kDa Saccharomyces cerevisiae protein
Buffer:	20 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6

Conformational Flexibility of Ubiquitin-Modified and SUMO-Modified PCNA Shown by Full-Ensemble Hybrid Methods. J Mol Biol 430(24):5294-5303 (2018)
Powers KT, Lavering ED, Washington MT

R_g^Guinier	4.7	nm
D_max	16.9	nm
Volume^Porod	310	nm³

SASDW89 – Proliferating Cell Nuclear Antigen (PCNA) sliding clamp

Proliferating cell nuclear antigen Kratky plot

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T

R_g^Guinier	3.4	nm
D_max	9.0	nm
Volume^Porod	137	nm³

SASDW99 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:30 µM

Proliferating cell nuclear antigenPCNA-associated factor experimental SAS data

Proliferating cell nuclear antigen PCNA-associated factor Kratky plot

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.5	nm
D_max	9.7	nm
Volume^Porod	128	nm³

SASDWA9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:60 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.5	nm
D_max	10.0	nm
Volume^Porod	158	nm³

SASDWB9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:90 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	184	nm³

SASDWC9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:150 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	219	nm³

SASDWD9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:200 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	208	nm³

SASDWE9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:300 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.9	nm
D_max	11.5	nm
Volume^Porod	194	nm³

SASDWF9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:370 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	187	nm³

SASDHQ9 – Proliferating cell nuclear antigen (PCNA) from pathogenic yeast Candida albicans

Proliferating cell nuclear antigen (C-terminal His-tagged) experimental SAS data

Sample:	Proliferating cell nuclear antigen (C-terminal His-tagged) trimer, 90 kDa Candida albicans protein
Buffer:	20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Sep 3

Structural analyses of PCNA from the fungal pathogen Candida albicans identify three regions with species-specific conformations. FEBS Lett (2021)
Sundaram R, Manohar K, Patel SK, Acharya N, Vasudevan D

R_g^Guinier	3.4	nm
D_max	10.2	nm
Volume^Porod	128	nm³

SASDBD7 – ...Proliferating Cell Nuclear Antigen (PCNA)

Sample:	Proliferating cell nuclear antigen trimer, 89 kDa Homo sapiens protein
Buffer:	25mM HEPES 100mM NaCl 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Jan 20

Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase. Nucleic Acids Res 45(5):2897-2909 (2017)
Prakash A, Moharana K, Wallace SS, Doublié S

R_g^Guinier	3.4	nm
D_max	9.7	nm
Volume^Porod	128	nm³

SASDBA7 – ...Proliferating Cell Nuclear Antigen (PCNA) and DNA

Endonuclease 8-like 1dsDNAProliferating cell nuclear antigen experimental SAS data

Sample:	Endonuclease 8-like 1 monomer, 45 kDa Homo sapiens protein dsDNA monomer, 2 kDa DNA Proliferating cell nuclear antigen monomer, 30 kDa Homo sapiens protein
Buffer:	25mM HEPES 100mM NaCl 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Jan 20

Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase. Nucleic Acids Res 45(5):2897-2909 (2017)
Prakash A, Moharana K, Wallace SS, Doublié S

R_g^Guinier	3.4	nm
D_max	16.4	nm
Volume^Porod	113	nm³

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