Small Angle Scattering Biological Data Bank SASBDB

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3 hits found for ST9 PKmutant (59 nt RNA)

SASDV25 – ST9a RNA, PKmutant (59 nucleotides in 0 mM MgCl2; monomer)

ST9 PKmutant (59 nt RNA) experimental SAS data

Sample:	ST9 PKmutant (59 nt RNA) monomer, 19 kDa RNA
Buffer:	50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH:
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30

A conserved viral RNA fold enables nuclease resistance across kingdoms of life. Nucleic Acids Res 53(16) (2025)
Gezelle JG, Korn SM, McDonald JT, Gong Z, Erickson A, Huang CH, Yang F, Cronin M, Kuo YW, Wimberly BT, Steckelberg AL

R_g^Guinier	2.5	nm
D_max	8.8	nm
Volume^Porod	29	nm³

SASDV35 – ST9a RNA, PKmutant (59 nucleotides in 0 mM MgCl2; dimer)

Sample:	ST9 PKmutant (59 nt RNA) dimer, 38 kDa RNA
Buffer:	50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH:
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30

R_g^Guinier	3.8	nm
D_max	12.5	nm
Volume^Porod	73	nm³

SASDVZ4 – ST9a RNA, PKmutant (59 nucleotides in 10 mM MgCl2; dimer)

Sample:	ST9 PKmutant (59 nt RNA) dimer, 38 kDa RNA
Buffer:	50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH:
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30

R_g^Guinier	3.5	nm
D_max	12.8	nm
Volume^Porod	62	nm³