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2 hits found for Thymine dioxygenase JBP1

SASDGP2 – Thymine dioxygenase full length J-DNA binding protein (JBP1)

Thymine dioxygenase JBP1 experimental SAS data
Thymine dioxygenase JBP1 Kratky plot
Sample: Thymine dioxygenase JBP1 monomer, 93 kDa Leishmania tarentolae protein
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 128 nm3

SASDGU2 – Thymine dioxygenase full length J-DNA binding protein in complex with J-23-DNA (JBP1:J-23-DNA)

Thymine dioxygenase JBP1J-DNA (23mer) experimental SAS data
Thymine dioxygenase JBP1 J-DNA (23mer) Kratky plot
Sample: Thymine dioxygenase JBP1 monomer, 93 kDa Leishmania tarentolae protein
J-DNA (23mer) monomer, 14 kDa DNA
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 4.1 nm
Dmax 14.1 nm
VolumePorod 148 nm3