Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly.

Kasiliauskaite A, Kubicek K, Klumpler T Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R, Nucleic Acids Res (2022) Europe PMC

SASDKZ9 – Transcription elongation factor SPT6 - ΔN Spt6

Transcription elongation factor SPT6 - ΔN Spt6 variant
MWexperimental 147 kDa
MWexpected 132 kDa
VPorod 288 nm3
log I(s) 6.87×103 6.87×102 6.87×101 6.87×100
Transcription elongation factor SPT6 - ΔN Spt6 variant small angle scattering data  s, nm-1
ln I(s)
Transcription elongation factor SPT6 - ΔN Spt6 variant Guinier plot ln 6.88×103 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
Transcription elongation factor SPT6 - ΔN Spt6 variant Kratky plot 1.104 0 √3 sRg
p(r)
Transcription elongation factor SPT6 - ΔN Spt6 variant pair distance distribution function Rg: 4.6 nm 0 Dmax: 14.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Transcription elongation factor SPT6 - ΔN Spt6 Rg histogram Rg, nm
log I(s)
 s, nm-1
Transcription elongation factor SPT6 - ΔN Spt6 variant CORAL model
Synchrotron SAXS data from solutions of Transcription elongation factor SPT6 - ΔN Spt6 in 25 mM Hepes; 150 NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.81 mg/ml was measured at 20°C. 20 successive 0.450 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Transcription elongation factor SPT6 - ΔN Spt6 variant (ΔN Spt6)
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Monomer
Mon. MW   132.4 kDa
 
UniProt   P23615
Sequence   FASTA
 
PDB ID   3PSI