Dsk2 Scattering Data

SASDYT2 – Full-length yeast shuttle protein Dsk2

Ubiquitin domain-containing protein DSK2
MWexperimental 40 kDa
MWexpected 39 kDa
log I(s) 6.43×101 6.43×100 6.43×10-1 6.43×10-2
Ubiquitin domain-containing protein DSK2 small angle scattering data  s, nm-1
ln I(s)
Ubiquitin domain-containing protein DSK2 Guinier plot ln 6.43×101 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Ubiquitin domain-containing protein DSK2 Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Full-length yeast shuttle protein Dsk2 in 20 mM NaPhos, 0.5 mM EDTA, 0.02% NaN3, pH 6.8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 6.1 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex KW-800 series column at 25°C. 600 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ubiquitin domain-containing protein DSK2 (DSK2)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Monomer
Mon. MW   39.3 kDa
 
UniProt   P48510 (1-373)
Sequence   FASTA