| MWexperimental | 68 | kDa |
| MWexpected | 85 | kDa |
| VPorod | 95 | nm3 |
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log I(s)
7.82×10-1
7.82×10-2
7.82×10-3
7.82×10-4
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s, nm-1
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Structural basis for therapeutic antibody binding to CCN2 and its mechanisms on TGF-β family signaling
Haben Gabir.SASDV79 – Mouse Central Communication Network 2 (CCN2) in complex with anti-CCN2 F(ab) antibody 37-45-MH1
Cellular communication network factor 2anti-CCN2 37-45-MH1 F(ab) antibody
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Fits and models
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Synchrotron SAXS
data from solutions of
Mouse Central Communication Network 2 (CCN2) in complex with anti-CCN2 F(ab) antibody 37-45-MH1
in
50 mM Tris pH 7.5, 200 mM NaCl, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.09537 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample
at 5 mg/ml was injected at a 1.60 ml/min flow rate
onto a column
at 15°C.
599 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SEC column = UNKNOWN |
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