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13 hits found for David Lambright

SASDCK7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399)

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 13
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
...Lambright DG
RgGuinier 2.8 nm
Dmax 11.2 nm
VolumePorod 61 nm3

SASDCL7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390)

Cytohesin-3 experimental SAS data
Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390) Rg histogram
Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.001 mM inositol 1,3,4,5-tetrakisphosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Mar 21
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
...Lambright DG
RgGuinier 2.8 nm
Dmax 9.3 nm
VolumePorod 64 nm3

SASDCM7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L fusion protein experimental SAS data
DAMMIF model
Sample: Grp1 63-399 E161A 6GS Arf6 Q67L fusion protein monomer, 61 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
...Lambright DG
RgGuinier 3.3 nm
Dmax 11.9 nm
VolumePorod 93 nm3

SASDCN7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L His6 fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein experimental SAS data
Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein Kratky plot
Sample: Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein monomer, 62 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
...Lambright DG
RgGuinier 3.4 nm
Dmax 13.6 nm
VolumePorod 101 nm3

SASDCP7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L SUMO fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L SUMO fusion protein experimental SAS data
DAMMIF model
Sample: Grp1 63-399 E161A 6GS Arf6 Q67L SUMO fusion protein monomer, 72 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
...Lambright DG
RgGuinier 3.8 nm
Dmax 14.5 nm
VolumePorod 116 nm3

SASDCQ7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein

Grp1 63-399 E161A Arf6 Q67L fusion protein experimental SAS data
Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein Rg histogram
Sample: Grp1 63-399 E161A Arf6 Q67L fusion protein monomer, 60 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
...Lambright DG
RgGuinier 3.1 nm
Dmax 11.6 nm
VolumePorod 86 nm3

SASDG64 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDG94 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDGA4 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models)

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDG74 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.3 nm
Dmax 25.7 nm
VolumePorod 168 nm3

SASDGB4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.1 nm
Dmax 25.7 nm
VolumePorod 168 nm3

SASDGC4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models)

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.1 nm
Dmax 25.7 nm
VolumePorod 168 nm3

SASDG84 – Autoinhibited dimer of truncated 6xHis Cytohesin-2 (ARNO, amino acids 2-400) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF, GASBOR and antiparallel CORAL models)

Cytohesin-2 experimental SAS data
DAMMIF model
Sample: Cytohesin-2 dimer, 95 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes
David Lambright
RgGuinier 5.3 nm
Dmax 27.0 nm
VolumePorod 180 nm3